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Noncanonical DNA polymerization by aminoadenine-based siphoviruses
Science ( IF 56.9 ) Pub Date : 2021-04-30 , DOI: 10.1126/science.abe6542
Valerie Pezo 1 , Faten Jaziri 1 , Pierre-Yves Bourguignon 2, 3 , Dominique Louis 4 , Deborah Jacobs-Sera 5 , Jef Rozenski 6 , Sylvie Pochet 7 , Piet Herdewijn 6 , Graham F. Hatfull 5 , Pierre-Alexandre Kaminski 8 , Philippe Marliere 1, 3
Affiliation  

Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3′-exonuclease but lacks 5′-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of Siphoviridae bacteriophages. Congruent phylogenetic clustering of the polymerases and biosynthesis enzymes suggests that aminoadenine has propagated in DNA alongside adenine since archaic stages of evolution.



中文翻译:

基于氨基腺嘌呤的siphoviruses非规范的DNA聚合

噬菌体基因组包含所有生命形式中最广泛的核碱基化学多样性。感染宿主的某些DNA病毒(例如蓝细菌,蛋白细菌和放线菌)表现出氨基腺嘌呤全面取代腺嘌呤的作用,从而与胸腺嘧啶形成三个氢键,并违反了Watson-Crick配对规则。与含有3'-核酸外切酶但缺少5'-核酸外切酶的细菌DNA聚合酶I的Klenow片段同源的氨基腺嘌呤编码的DNA聚合酶被发现在以胸腺嘧啶为模板的脱氧核苷三磷酸掺入中优先选择氨基腺嘌呤而不是腺嘌呤。聚合酶基因与生物合成酶的基因同时发生,该生物合成酶在各种各样的甲型流感病毒科中产生氨基腺嘌呤脱氧核苷酸噬菌体。聚合酶和生物合成酶的系统同源聚类表明,自古进化阶段以来,氨基腺嘌呤就与腺嘌呤一起在DNA中繁殖。

更新日期:2021-04-30
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