The interrelationships and synergistic regulations of bioactive molecules play pivotal roles in physiological and pathological processes involved in the initiation and development of some diseases, such as cancer and neurodegenerative and cardiovascular diseases. Therefore, the simultaneous, accurate and timely detection of two bioactive molecules is crucial to explore their roles and pathological mechanisms in related diseases. Fluorescence imaging associated with small molecular probes has been widely used in the imaging of bioactive molecules in living cells and in vivo due to its excellent performances, including high sensitivity and selectivity, noninvasive properties, real-time and high spatial temporal resolution. Single organic molecule fluorescent probes have been successively developed to simultaneously monitor two biomolecules to uncover their synergistic relationships in living systems. Hence, in this review, we focus on summarizing the design strategies, classifications, and bioimaging applications of dual-response fluorescent probes over the past decade. Furthermore, future research directions in this field are proposed.

生物活性分子的相互关系和协同调控在涉及某些疾病（例如癌症，神经退行性疾病和心血管疾病）的发生和发展的生理和病理过程中起着关键作用。因此，同时，准确和及时地检测两种生物活性分子对于探索它们在相关疾病中的作用和病理机制至关重要。与小分子探针相关的荧光成像已广泛用于活细胞和体内生物活性分子的成像由于其出色的性能，包括高灵敏度和选择性，无创性，实时和高空间时间分辨率。单个有机分子荧光探针已经相继开发，可以同时监测两个生物分子以揭示它们在生命系统中的协同关系。因此，在这篇综述中，我们着重于总结过去十年中双响应荧光探针的设计策略，分类和生物成像应用。此外，提出了该领域的未来研究方向。