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Light-driven CO2 sequestration in Escherichia coli to achieve theoretical yield of chemicals
Nature Catalysis ( IF 37.8 ) Pub Date : 2021-04-29 , DOI: 10.1038/s41929-021-00606-0
Guipeng Hu , Zehong Li , Danlei Ma , Chao Ye , Linpei Zhang , Cong Gao , Liming Liu , Xiulai Chen

CO2 sequestration engineering is an attractive strategy for achieving carbon- and energy-efficient bioproduction. However, the efficiency of heterotrophic CO2 sequestration is limited by bioproduct dependence and energy deficiency. Here, modular CO2 sequestration engineering was developed to produce target chemicals by integrating synthetic CO2 fixation and CO2 mitigation modules. A synthetic CO2 fixation pathway was designed, and then enhanced by light-driven reducing power using self-assembled cadmium sulfide nanoparticles. Next, a CO2 mitigation switch was designed, and then optimized by light-driven energy via proteorhodopsin. Finally, by integrating CO2 fixation and CO2 mitigation modules, the efficiency of CO2 sequestration was notably enhanced in Escherichia coli and the yields of l-malate and butyrate were increased to 1.48 and 0.79 mol/mol glucose, respectively, reaching theoretical yields. This CO2 sequestration system provides an efficient platform for channelling CO2 into value-added chemicals.



中文翻译:

在大肠杆菌中光驱动 CO2 封存以实现化学品的理论产量

CO 2封存工程是实现碳和能源高效生物生产的一种有吸引力的策略。然而,异养CO 2封存的效率受到生物产品依赖性和能量缺乏的限制。在这里,开发了模块化 CO 2封存工程,通过集成合成 CO 2固定和 CO 2缓解模块来生产目标化学品。设计了一种合成的 CO 2固定途径,然后使用自组装硫化镉纳米粒子通过光驱动还原能力增强。接下来,设计了一个 CO 2缓解开关,然后通过原视紫红质通过光驱动能量进行优化。最后,通过整合 CO2固定和CO 2减缓模块,CO 2封存效率在大肠杆菌中显着提高,L-苹果酸和丁酸的产率分别提高到1.48和0.79 mol/mol葡萄糖,达到理论产率。该 CO 2封存系统为将 CO 2转化为增值化学品提供了一个有效平台。

更新日期:2021-04-29
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