Coxiella burnetii (C. burnetii) is a bacterial agent causing Q fever which is widespread all over the world. Livestock such as cattle, goat, and sheep are the main sources of infection for this disease. Infection of C. burnetii causes abortion of livestock, resulting in economic damage. Q fever is zoonotic disease and potential public health hazard. To date, little is known about the infection of C. burnetii in livestock in Indonesia. The objective of this research is to screen the genome of C. burnetii bacteria in beef cattle in West Java, Indonesia. Organ tissue samples were collected from cattle slaughtered in slaughterhouses, West Java. C. burnetii genome was detected from cattle samples in all three samplings area by nested PCR (nPCR) targeting com1 gene of C. burnetii. Sequencing analysis of 16S rRNA gene revealed the amplicons showed 99.9% nucleotide identity to C. burnetii strains Heizberg, 1843, 2574, 701CbB1, and 14160-001. Our results indicate that the infection of C. burnetii occurs in Indonesian beef cattles and highlight the risk of exposure to C. burnetii infection in human.

Coxiella burnetii ( C. burnetii ) 是一种引起 Q 热的细菌，在世界各地都很普遍。牛、山羊和绵羊等牲畜是该病的主要感染源。C. burnetii感染导致牲畜流产，造成经济损失。Q热是人畜共患病和潜在的公共卫生危害。迄今为止，关于印度尼西亚牲畜感染C. burnetii的情况知之甚少。本研究的目的是筛选印度尼西亚西爪哇肉牛中伯氏梭菌的基因组。 器官组织样本是从西爪哇屠宰场屠宰的牛身上收集的。C.burnetii采用巢式PCR（nPCR）靶向C. burnetii com1基因，从三个采样区的牛样本中检测到基因组。16S rRNA 基因的测序分析显示，这些扩增子与C. burnetii菌株 Heizberg、1843、2574、701CbB1 和 14160-001 具有 99.9% 的核苷酸同一性。我们的研究结果表明C. burnetii感染发生在印度尼西亚肉牛中，并突出了人类接触C. burnetii感染的风险。