The accelerating decline of pollinator populations has become increasingly concerning in the last two decades. Honey bees are economically important pollinators and a model species to evaluate pollinator health. The decline in queen quality is one of the frequently reported causes associated with honey bee losses worldwide. Histopathology is an essential tool used for diagnostics and research in mammalian species and may provide insight into the histomorphological basis of a decline in queen quality. Thus, the purpose of this study was to summarize the previously described normal morphology of the entire reproductive tract of mated honey bee queens and to illustrate it by high quality photomicrographs. Accordingly, reproductive tracts of one-year old mated honey bee queens were processed for histology, serially sectioned, stained with hematoxylin and eosin, and used for the capture of high quality histological photomicrographs of the entire reproductive tract. This study illustrates the microscopic morphology of the queen reproductive tract which may facilitate further investigations of the cause and pathogenesis of the recent decline in reproductive fitness of honey bees using histopathology.

在过去的二十年中，传粉媒介种群的加速下降变得越来越令人担忧。蜜蜂是经济上重要的传粉媒介，也是评估传粉媒介健康的模式物种。蜂王质量下降是世界范围内与蜜蜂损失有关的常见原因之一。组织病理学是用于哺乳动物物种诊断和研究的重要工具，可以深入了解蜂王质量下降的组织形态学基础。因此，本研究的目的是总结先前描述的已交配的蜜蜂蜂王整个生殖道的正常形态，并通过高质量的显微照片进行说明。因此，对一岁大的交配蜂王的生殖道进行组织学处理，连续切片，用苏木精和伊红染色，用于捕获整个生殖道的高质量组织学显微照片。这项研究说明了蜂王生殖道的微观形态，这可能有助于使用组织病理学进一步研究蜜蜂最近生殖健康下降的原因和发病机制。