A reduced graphite oxide nanosheet electrode (RGOnS) was prepared as a sensor for amoxicillin (AMX) detection, an antibiotic commonly used in the livestock farm, by the square-wave adsorptive stripping voltammetry technique. Graphite oxide with nanosheet shape was produced from a graphite electrode by a chronoamperometry process at 5 V and then an electrochemical reduction process was carried out to form RGOnS with restored long-range conjugated networks and better conductivity. The electrodes were characterized by SEM, EDX, and FTIR spectroscopy. The RGOnS electrode prepared at an optimal reduction potential of −1 V for 120 s exhibits a larger electrochemical active surface area, and the obtained oxidation signal of AMX is approximately ten times higher than that of the pristine graphite electrode. The analytical conditions such as the pH of electrolyte and accumulation time were optimized. The calibration curve built under the optimal conditions provided a good linear relationship in the range of AMX concentration from 0.5–80 µM with the correlation coefficient of 0.9992. The limit of detection was calculated as 0.193 µM. Satisfactory results are obtained from the detection of the AMX in different samples using the prepared electrode.

中文翻译：

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还原性氧化石墨纳米片电极伏安法测定阿莫西林

通过方波吸附溶出伏安法制备了还原型氧化石墨纳米片电极（RGOnS），作为用于阿莫西林（AMX）检测的传感器，阿莫西林是畜牧场中常用的抗生素。通过计时电流法在5 V下从石墨电极生产出具有纳米片状形状的氧化石墨，然后进行电化学还原过程以形成具有恢复的长距离共轭网络和更好的导电性的RGOnS。通过SEM，EDX和FTIR光谱对电极进行表征。在-1 V的最佳还原电位下持续120 s制备的RGOnS电极表现出更大的电化学活性表面积，并且所获得的AMX氧化信号约为原始石墨电极的十倍。优化了分析条件，例如电解质的pH值和累积时间。在最佳条件下建立的校准曲线在0.5–80的AMX浓度范围内提供了良好的线性关系。 µ M，相关系数为0.9992。计算检测限为0.193  μ M.令人满意的结果从使用制备的电极在不同样品中检测到的AMX获得。