Ticks will diminish productivity among farm animals and transmit zoonotic diseases. We conducted a study to identify tick species infesting slaughter bulls from Adama City and to screen them for tick-borne pathogens. In 2016, 291 ticks were collected from 37 bulls in Adama, which were ready for slaughter. Ticks were identified morphologically. Total genomic DNA was extracted from ticks and used to test for Rickettsia spp. with real-time PCR. Species identification was done by phylogenetic analysis using sequencing that targeted the 23S-5S intergenic spacer region and ompA genes. Four tick species from two genera, Amblyomma and Rhipicephalus, were identified. Amblyomma cohaerens was the dominant species (n = 241, 82.8%), followed by Amblyomma variegatum (n = 22, 7.5%), Rhipicephalus pulchellus (n = 19, 6.5%), and Rhipicephalus decoloratus (n = 9, 3.0%). Among all ticks, 32 (11%) were positive for Rickettsia spp. and 15 (5.2%) of these were identified as R. africae comprising at least two genetic clades, occurring in A. variegatum (n = 10) and A. cohaerens (n = 5). The remainder of Rickettsia-positive samples could not be amplified due to low DNA yield. Furthermore, another 15 (5.2%) samples carried other pathogenic bacteria: Ehrlichia ruminantium (n = 9; 3.1%) in A. cohaerens, Ehrlichia sp. (n = 3; 1%) in Rh. pulchellus and A. cohaerens, Anaplasma sp. (n = 1; 0.5%) in A. cohaerens, and Neoehrlichia mikurensis (n = 2; 0.7%) in A. cohaerens. All ticks were negative for Bartonella spp., Babesia spp., Theileria spp., and Hepatozoon spp. We reported for the first time E. ruminatium, N. mikurensis, Ehrlichia sp., and Anaplasma sp. in A. cohaerens. Medically and veterinarily important pathogens were mostly detected from A. variegatum and A. cohaerens. These data are relevant for a One-health approach for monitoring and prevention of tick-borne disease transmission.

壁虱会降低家畜的生产力，并传播人畜共患疾病。我们进行了一项研究，以鉴定出来自阿达玛市的屠宰公牛的tick物种，并筛选它们的tick传播病原体。2016年，从阿达玛的37头公牛身上收集了291头s子，准备宰杀。虫在形态上被鉴定。从tick中提取总基因组DNA，并用于检测立克次体。实时PCR。通过系统发育分析，使用针对23S-5S基因间隔区和ompA基因的测序进行物种鉴定。鉴定出了两个属中的四个tick种，分别是盲mb和蛇脑。羊膜菌是优势种（n = 241，82.8％），其次是杂草盲草（n = 22，7.5％），phipchelcephalus pulchellus（n = 19，6.5％）和Rhipicephalus decoloratus（n = 9，3.0％）。在所有壁虱中，立克次体属呈阳性的占32个（11％）。其中15个（5.2％）被鉴定为非洲曲霉，包含至少两个遗传进化枝，分别发生在杂色曲霉（n = 10）和co。cohaerens（n = 5）中。由于DNA产量低，其余立克次体阳性样品无法扩增。此外，另外15个（5.2％）样本携带其他病原细菌：沙眼衣原体（n = 9; 3.1％）A. cohaerens，埃里希氏菌。（n = 3; 1％）在Rh中。pulchellus和A. cohaerens，Anaplasma sp。（N = 1; 0.5％）在A. cohaerens，和Neoehrlichia mikurensis（N = 2; 0.7％）在A. cohaerens。所有的蜱负巴尔通体属，巴贝虫属，泰勒虫属，和Hepatozoon属。我们报告首次E. ruminatium，N. mikurensis，埃立克体属，无形体属。在A. cohaerens中。在医学和兽医学上重要的病原体大多从杂色曲霉和co。cohaerens中检出。这些数据与监测和预防tick传播疾病传播的“一站式”方法有关。