Tef is the most important and well adapted cereal crop in Ethiopia and grows better than other cereals both in biotic and abiotic stress conditions. In this study 93 tef accessions representative of various agro-ecologies of Ethiopia along with four improved varieties, one local check and six wild relatives were studied. The materials were genotyped with 12 simple sequence repeat markers (SSRs) to investigate the extent and structure of the genetic diversity of tef. A total of 152 alleles were detected indicating high polymorphism in the studied accessions. The number of alleles per polymorphic loci ranged from seven for CNLT 538 to 29 for CNLT150 with an average of 12.67. High average polymorphic information content (0.82) and average expected heterozygosity (0.86) also signifies the suitability and efficiency of SSR markers to discriminate among tef genotypes. Cluster, principal coordinate and structure analyses grouped the 98 tef genotypes and six wild relatives into four distinct classes. The groupings showed no special pattern despite the different regions of origins for the accessions; however, the five wild relatives were grouped together. The analysis of molecular variance and fixation index showed low genetic differentiation and high gene flow between regions revealing that much of the variability was attributed to within populations variation. Generally, the present study showed that there is high genetic variability with unstructured genetic pattern among the studied tef genotypes which implies the vitality of conserved tef germplasms at Ethiopian Biodiversity Institute and the impact of the seed exchange system on tef breeding activities in Ethiopia.

Tef是埃塞俄比亚最重要且适应性最强的谷类作物，在生物和非生物胁迫条件下，Tef的生长均优于其他谷物。在这项研究中，研究了代表埃塞俄比亚各种农业生态的93种特氟登种质，以及四个改良品种，一个当地对照和六个野生亲缘种。将这些材料与12个简单序列重复标记（SSR）进行基因分型，以研究tef遗传多样性的程度和结构。总共检测到152个等位基因，表明在所研究的种质中具有高多态性。每个多态性基因座的等位基因数量范围从CNLT 538的7个到CNLT150的29个，平均为12.67。高平均多态信息含量（0.82）和平均预期杂合度（0。86）还表示SSR标记在tef基因型之间进行区分的适用性和效率。聚类，主要坐标和结构分析将98个tef基因型和6个野生亲戚分为四个不同的类别。尽管这些种质的原产地不同，但这些分类没有显示出特殊的模式。但是，五个野生亲戚被归为一类。分子变异和固定指数的分析表明，区域之间的遗传分化较低，基因流动较高，这表明许多变异是由于种群内部变异造成的。一般来说，