In the present study, the digestive enzyme activity during larval development in L. peru reared under intensive culture conditions was evaluated. The results revealed that L. peru larvae have the necessary enzymes to digest proteins, lipids and carbohydrates when nutritional reserves have been depleted at the onset of the exogenous feeding at 3 days after hatching (DAH) or 78 degree-days (DD). The activities of the digestive enzymes follow patterns similar to those previously reported in other related species. As in other marine fish larvae, the amylase specific activity decreased during larval development of L. peru. At 25 DAH (650 DD), the specific activities of lipase, alkaline phosphatase and acid phosphatase declined, while the specific activities of pepsin, trypsin and chymotrypsin increased. In contrast, the individual activity of all enzymes steadily increased with development. The time required to reach the maximum activity varies, suggesting a relative importance of different digestive enzymes during the different developmental stages of the digestive tract. In our study, the highest activity was reached by amylase at Stage I and early Stage II (3–11 DAH or 78–286 DD), by lipase and both acid and alkaline phosphatases at the end of Stage II (18–22 DAH or 468–572 DD), and by trypsin, chymotrypsin and acid protease at Stage III (25 DAH or 650 DD). This study provides new data on the digestive physiology of larval L. peru that are particularly pertinent to early weaning and nutritional physiology, and that may be useful in the larval culture of this species.

中文翻译：

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养殖太平洋红鲷鱼幼体发育过程中消化酶活性的变化

在本研究中，L .幼虫发育过程中的消化酶活性。 对在精养条件下饲养的秘鲁进行了评估。结果表明，L . 当在孵化后 3 天 (DAH) 或 78 度日 (DD) 开始外源喂养时营养储备耗尽时，秘鲁幼虫具有消化蛋白质、脂质和碳水化合物所需的酶。消化酶的活动遵循与先前在其他相关物种中报道的相似的模式。与其他海鱼幼虫一样，在L幼虫发育过程中淀粉酶比活性降低。 秘鲁. 在25 DAH（650 DD），脂肪酶、碱性磷酸酶和酸性磷酸酶的比活性下降，而胃蛋白酶、胰蛋白酶和糜蛋白酶的比活性升高。相比之下，所有酶的个体活性随着发育而稳步增加。达到最大活性所需的时间各不相同，这表明不同消化酶在消化道不同发育阶段的相对重要性。在我们的研究中，淀粉酶在 I 期和 II 期早期（3-11 DAH 或 78-286 DD）达到最高活性，II 期末（18-22 DAH 或 78-286 DD）脂肪酶和酸性和碱性磷酸酶达到最高活性。 468–572 DD），以及在 III 期（25 DAH 或 650 DD）通过胰蛋白酶、胰凝乳蛋白酶和酸性蛋白酶。这项研究为L幼虫的消化生理提供了新数据. peru与早期断奶和营养生理学特别相关，并且可能在该物种的幼虫培养中有用。