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Titanium Dioxide Nanoparticles Increase Tissue Ti Concentration and Activate Antioxidants in Solanum lycopersicum L.
Journal of Soil Science and Plant Nutrition ( IF 3.9 ) Pub Date : 2021-04-28 , DOI: 10.1007/s42729-021-00487-z
Ricardo Tighe-Neira , Marjorie Reyes-Díaz , Adriano Nunes-Nesi , Gonzalo Recio , Erico R. Carmona , Ricard Marcos , Alexandre Corgne , Zed Rengel , Claudio Inostroza-Blancheteau

This work was aimed at characterizing the effects of foliarly applied rutile (TiO2) nanoparticles (NPs) on Ti translocation as well as biomass production and antioxidant system in tomato (Solanum lycopersicum L.). The seeds were germinated and grown on a substrate in individual pots in a growth chamber. The TiO2 NPs were characterized using transmission electron microscopy (TEM), Raman spectroscopy, dynamic light scattering (DLS), and laser doppler velocimetry (LDV). Titanium dioxide NPs had a rod-shaped form and were moderately prone to agglomeration. The TiO2 NPs treatments were applied at 0, 5, 10, 20, 40, 80, and 160 mg L−1 by foliar spraying on 20-day-old S. lycopersicum plants. After 7 days of treatment exposure, tissue Ti concentration was determined by inductively coupled plasma-mass spectrometry (ICP-MS). Nanoparticle treatments increased tissue Ti concentration; Ti was translocated from leaves to stem, triggering a significant decrease in biomass production. With respect to the control, an increase in total reducing capacity (1.7-fold), antioxidant activity (1.5-fold), and superoxide dismutase activity (2-fold) were observed in the treatments with intermediate to high doses. The TiO2 NPs triggered an increase in tissue Ti concentration, increasing the antioxidant system activity and lipid peroxidation at low to intermediate doses, and decreasing biomass production at intermediate to high doses.



中文翻译:

钛白粉纳米颗粒可提高茄果茄中组织Ti的浓度并激活抗氧化剂。

这项工作旨在表征叶面施用的金红石(TiO 2)纳米颗粒(NPs)对番茄(Solanum lycopersicum L.)的Ti转运以及生物量产生和抗氧化系统的影响。种子发芽并在生长室中各个盆中的基质上生长。使用透射电子显微镜(TEM),拉曼光谱,动态光散射(DLS)和激光多普勒测速仪(LDV)对TiO 2 NP进行表征。二氧化钛纳米颗粒呈棒状,中等程度的团聚。TiO 2 NPs处理通过在20日龄的番茄上进行叶面喷洒分别以0、5、10、20、40、80和160 mg L -1进行植物。治疗暴露7天后,通过电感耦合等离子体质谱法(ICP-MS)测定组织Ti浓度。纳米粒子处理增加了组织中的钛浓度;钛从叶转移到茎,触发生物量生产的显着减少。关于对照,在中至高剂量的治疗中观察到总还原能力(1.7倍),抗氧化剂活性(1.5倍)和超氧化物歧化酶活性(2倍)的增加。TiO 2 NPs触发了组织Ti浓度的增加,中低剂量时增加了抗氧化系统的活性和脂质过氧化作用,中高剂量时则减少了生物量的产生。

更新日期:2021-04-29
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