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The modes of action of Mentha arvensis essential oil on the granary weevil Sitophilus granarius revealed by a label-free quantitative proteomic analysis
Journal of Pest Science ( IF 4.8 ) Pub Date : 2021-04-28 , DOI: 10.1007/s10340-021-01381-4
François Renoz , Sébastien Demeter , Hervé Degand , Stamatios C. Nicolis , Olivier Lebbe , Henri Martin , Jean‐Louis Deneubourg , Marie-Laure Fauconnier , Pierre Morsomme , Thierry Hance

Although synthetic pesticides are still used to control insect pests, greater efforts have been made to develop healthier and more environmentally friendly pesticides. Because of their insecticidal properties, botanical essential oils (EOs) are considered as promising alternatives to the use of synthetic insecticides. However, little is known about mechanisms underlying the insecticidal activity of most these natural compounds. In the present study, we evaluated the contact toxicity and the modes of action of the EO from Mentha arvensis against the granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae), a cosmopolitan insect pest that causes extensive damage to stored cereals. M. arvensis EO caused high contact toxicity in S. granarius adults, resulting in a rapid paralysis and rapid alteration of walking behavior. Our label-free quantitative proteomics approach revealed that M. arvensis EO induced dramatic physiological changes in exposed insects. The majority of the differentially expressed proteins (DEPs) were upregulated and are related to the development and functioning of the muscular and nervous systems, cellular respiration, protein synthesis, and detoxification. These results suggest that M. arvensis essential oil is capable of affecting a variety of biological processes, and shed light on the repair mechanisms put in place in surviving insects to counter the damage inflicted. This work opens new perspectives on the proposed mechanisms of insecticidal activity of a promising EO for controlling pests of stored cereals and may represent a first step in the development of novel bio-rational insecticides.



中文翻译:

无标记定量蛋白质组学分析揭示了薄荷脑香精油对谷类象鼻虫Sitophilus granarius的作用方式

尽管仍然使用合成农药来控制害虫,但是人们已经做出了更大的努力来开发更健康,更环保的农药。由于其杀虫特性,植物精油(EOs)被认为是合成杀虫剂使用的有前途的替代品。然而,对于大多数这些天然化合物的杀虫活性的潜在机理了解甚少。在本研究中,我们评估了薄荷脑中的EO对谷类象鼻虫Sitophilus granarius L.(鞘翅目:Curculionidae)的接触毒性和作用方式,这是一种大都会害虫,会对储存的谷物造成广泛破坏。M. arvensis EO引起高接触毒性。S. granarius成虫,导致快速瘫痪和步行行为的快速改变。我们的无标签定量蛋白质组学方法显示,M。arvensis EO导致暴露的昆虫发生显着的生理变化。大多数差异表达的蛋白质(DEP)均被上调,并与肌肉和神经系统的发育和功能,细胞呼吸,蛋白质合成和排毒有关。这些结果表明M. arvensis精油能够影响多种生物过程,并阐明了存活的昆虫为抵御所造成的损害而采取的修复机制。这项工作为控制贮藏谷物的有害生物的有前景的EO的拟议杀虫活性机理提出了新的观点,并且可能代表了新型生物合理杀虫剂开发的第一步。

更新日期:2021-04-29
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