The development of silver nanoparticles has attracted great attention due to their potential applications in biomedicine, tissue engineering and materials science. In this study, polyphenol coated silver nanoparticles (PAgNp) were synthesized using yerba mate (Ilex paraguariensis) aqueous extract as green reducing and stabilizing agent. This one-pot and economic process lead to a potent antimicrobial and antioxidant nanomaterial, both activities strongly essential for tissue engineering and wound healing. Yerba mate dried leaves are highly available and inexpensive industrial product in South America and other countries. Yerba mate plant aqueous extract was used as a reducing reactant and stabilizer agent, due its great content of phenolic compounds. Different parameters (i.e.: silver salt and reducing agent concentration, pH, temperature and capping agent concentration) were evaluated to establish the optimal synthesis condition. The reaction was evaluated by UV–vis spectrophotometry. Reaction efficiency (98%) and stability were measured by atomic absorption spectroscopy. The green synthesized nanomaterial was characterized by transmission electron microscopy (TEM), Dynamic Light Scattering (DLS), Energy-dispersive X-ray spectroscopy (EDS) and ATR-FTIR. Moreover, antimicrobial, antioxidant and cytocompatibility test were performed. The present study revealed that the low-cost, simple and environmentally friendly synthesis of a nanomaterial that can be widely used in biomedicine and nanobiotechnology as an antimicrobial and antioxidant agent.

银纳米颗粒的开发由于其在生物医学，组织工程和材料科学中的潜在应用而备受关注。在这项研究中，多酚涂层的银纳米粒子（PAgNp）是使用yerba mate（巴拉圭Ilex）合成的。）水提取物作为绿色还原和稳定剂。这种一站式的经济过程导致了一种有效的抗微生物和抗氧化剂纳米材料，这两种活性对于组织工程和伤口愈合都至关重要。在南美洲和其他国家/地区，Yerba mate干叶是高价且便宜的工业产品。Yerba mate植物水提取物由于其酚类化合物含量高而被用作还原剂和稳定剂。评价了不同的参数（即：银盐和还原剂的浓度，pH，温度和封端剂的浓度）以建立最佳合成条件。通过UV-可见分光光度法评价反应。反应效率（98％）和稳定性通过原子吸收光谱法测量。通过透射电子显微镜（TEM），动态光散射（DLS），能量色散X射线光谱（EDS）和ATR-FTIR对绿色合成纳米材料进行了表征。此外，进行了抗微生物，抗氧化剂和细胞相容性测试。本研究表明，低成本，简单且环保的纳米材料合成可以广泛用作生物医学和纳米生物技术中的抗微生物剂和抗氧化剂。