Whole-genome sequence of Pseudomonas sp. Kongs-67 retrieved from Kongsfjorden, an Arctic fjord, has been investigated to understand the molecular machinery required for microbial association and survival in a polar fjord. The genome size of Kongs-67 was 4.5 Mb and was found to be closely related to the Antarctic P. pelagia strain CL-AP6. This genome encodes for chemotaxis response regulator proteins (CheABB1RR2VWYZ), chemoreceptors (methyl-accepting chemotaxis proteins), and flagellar system proteins (FliCDEFGOPMN, FlhABF, FlgBCDEFGHIJKL, and MotAB proteins) vital in cellular interactions in the dynamic fjord environment. A high proportion of genes were assigned to biofilm formation (pgaABCD operon) and signal transduction protein categories (EnvZ/OmpR, CpxA/CpxR, PhoR/PhoB, PhoQ) indicating that the biofilm formation in Kongs-67 could be tightly regulated in response to the availability of signalling-metabolites. The genome of Kongs-67 encoded for HemBCD, CbiA, CobABNSTOQCDP, and BtuBFR proteins involved in cobalamin biosynthesis and transport along with proteins for siderophore-mediated iron channelling (PchR, Fur protein, FpvA); crucial in a microbial association. The genomes of Arctic strain Kongs-67 and Antarctic strain CL-AP6 were similar which is indicative of retainment of the core genes in the polar Pseudomonas strains that could be vital in conferring evolutionary adaptation for its survival in a polar fjord. Thus, our study contributes to the knowledge on the genetics of a polar Pseudomonas member exhibiting biosynthetic potentials and suggest Pseudomonas sp. Kongs-67 as a suitable candidate for the investigation of functional aspects of molecular adaptations in the polar marine environment.

假单胞菌sp。的全基因组序列。已经对从北极峡湾的Kongsfjorden回收的Kongs-67进行了研究，以了解在极地峡湾中微生物缔合和生存所需的分子机制。Kongs-67的基因组大小为4.5 Mb，发现与南极P. pelagia菌株CL-AP6密切相关。该基因组编码趋化性反应调节蛋白（CheABB1RR2VWYZ），趋化感受器（甲基接受趋化蛋白）和鞭毛系统蛋白（FliCDEFGOPMN，FlhABF，FlgBCDEFGHIJKL和MotAB蛋白）在动态峡湾环境中的细胞相互作用中至关重要。高比例的基因被分配给生物膜形成（pgaABCD操纵子）和信号转导蛋白类别（EnvZ / OmpR，CpxA / CpxR，PhoR / PhoB，PhoQ）表明，Kongs-67中的生物膜形成可能受到信号代谢物可用性的严格调控。Kongs-67的基因组编码HemBCD，CbiA，CobABNSTOQCDP和BtuBFR蛋白，参与钴胺素的生物合成和运输，以及铁载体介导的铁通道蛋白（PchR，Fur蛋白，FpvA）；在微生物关联中至关重要。北极菌株Kongs-67和南极菌株CL-AP6的基因组相似，这表明极性假单胞菌菌株中核心基因的保留，这对于赋予进化适应性使其在极地峡湾中的生存至关重要。因此，我们的研究有助于了解极地遗传学假单胞菌成员表现出生物合成的潜力，并建议假单胞菌sp。Kongs-67是研究极地海洋环境中分子适应功能方面的合适人选。