Tissue engineering in reconstructive surgery seeks to generate bioartificial tissue substitutes. The arteriovenous (AV) loop allows the generation of axially vascularized tissue constructs. Cellular mechanisms of this vascularization process are largely unclear. In this study, we developed two different chamber models for intravital microscopy and imaging of the AV loop in the rat. Multiple design variations were implanted and the stability of the chamber and AV loop patency was tested in vivo. Our novel chamber facilitates repetitive observation of the AV loop using fluorescence-enhanced intravital microscopy. This technique can be used for daily evaluation of leukocyte-endothelial cell interactions, vascularization, and tissue formation in the AV loop model on 14 consecutive days. Therefore, our newly developed model for intravital microscopy will provide better understanding of cellular and molecular processes in tissue engineering in the AV loop. Moreover, it supports initiation of the novel approaches for therapeutic applications.

中文翻译：

---

观察血管生长：在动静脉环大鼠模型中建立活体显微镜

重建手术中的组织工程旨在生成生物人工组织替代品。动静脉 (AV) 环允许产生轴向血管化组织结构。这种血管化过程的细胞机制在很大程度上尚不清楚。在这项研究中，我们开发了两种不同的腔室模型，用于活体显微镜检查和大鼠 AV 环成像。植入了多种设计变体，并在体内测试了腔室的稳定性和 AV 环路的通畅性. 我们的新型腔室有助于使用荧光增强活体显微镜重复观察 AV 环。该技术可用于连续 14 天对 AV 环模型中白细胞-内皮细胞相互作用、血管化和组织形成的日常评估。因此，我们新开发的活体显微镜模型将更好地了解AV 循环中组织工程中的细胞和分子过程。此外，它支持启动治疗应用的新方法。