Aims

This study aimed to better understanding the mechanism underlying the interaction between boron and NaCl and may provide methods to improve sugar beet production in saline soils.

Methods

Sugar beet seedlings were treated with different concentrations of boron and NaCl. The flame photometer and ion chromatography were used to determine the ion content. And the uptake of Cl⁻ by beet roots, was measured by non-invasive micro-test technology (NMT) and in vivo uptake system. The qPCR was used to measure the relative expression of the genes encoding ion transporters.

Results

In this study, we demonstrate that application of 750 µM boron markedly mitigated the growth inhibition of sugar beet seedlings caused by 300 mM NaCl, as reflected in dry and fresh weight, leaf area, chlorophyll content, and net photosynthetic rates. Both Na⁺ and K⁺ in the plants were not affected by treatment with boron. However, boron markedly reduced the Cl⁻ content of sugar beet seedlings. Boron profoundly upregulated the expression of CLC-b, CLC-c, CLC-f and downregulated the expression of NIP5, NIP6 and AKT1 under NaCl stress.

Conclusions

Our results indicate that exogenous boron reduced Cl⁻ content of sugar beet seedlings by regulation of Cl⁻ transport under NaCl stress, which is possibly involved in upregulation of BvCLC-b, BvCLC-c and BvCLC-f genes encoding Cl⁻ transporters in a cell wall-dependent Ca²⁺ signal transduction process.

中文翻译：

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外源硼通过减少甜菜中的Cl-吸收减轻了NaCl胁迫的生长抑制作用（甜菜）

目的

这项研究旨在更好地了解硼与NaCl之间相互作用的机理，并可能提供改善盐渍土壤中甜菜产量的方法。

方法

用不同浓度的硼和氯化钠处理甜菜幼苗。使用火焰光度计和离子色谱法确定离子含量。和Cl的摄取^-由甜菜根，通过非侵入性的微测试技术（NMT）和测量在体内吸收系统。qPCR用于测量编码离子转运蛋白的基因的相对表达。

结果

在这项研究中，我们证明了750 µM硼的施用显着减轻了300 mM NaCl引起的甜菜幼苗的生长抑制，这反映在干重和鲜重，叶面积，叶绿素含量和净光合速​​率上。植物中的Na ⁺和K ⁺均不受硼处理的影响。然而，硼显着减少的氯^-糖甜菜幼苗内容。在NaCl胁迫下，硼显着上调了CLC-b，CLC-c，CLC-f的表达，并下调了NIP5，NIP6和AKT1的表达。

结论

我们的研究结果表明，外源硼降低氯^-经Cl调控糖用甜菜幼苗内容^-盐胁迫，其可能参与上调下运输BvCLC-B ，BvCLC-C和BvCLC-F基因编码氯^-在细胞中转运依赖于壁的Ca ²⁺信号转导过程。