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Quantification of Phenolics from Coriandrum sativum vulgare and Coriandrum sativum microcarpum by HPLC–DAD
Iranian Journal of Science and Technology, Transactions A: Science ( IF 1.7 ) Pub Date : 2021-04-27 , DOI: 10.1007/s40995-021-01132-1
Kadriye Ozlem Saygi

Phenolic compounds found in the human diet reveal a broad spectrum of biological activities. The present work describes the quantitative analysis of phenolic compounds in Coriandrum sativum vulgare (Csv) and Coriandrum sativum microcarpum (Csm) by HPLC–DAD analyses. The methanolic extracts of both varieties were analyzed by reverse phase HPLC. The method was validated in terms of linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and repeatability, as well. The coefficient of determination (R2) was in the range of 0.9986–0.9993. The LOD and LOQ ranged from 1.13–1.46 to 3.42–4.32 mg/kg, respectively. The total phenolic contents of both varieties were also presented using Folin–Ciocalteu method with gallic acid equivalent in mg g−1 dried weight. Quercetin-3-β-D glucoside (Q3GD) (103.81 \(\pm \) 4.76 mg kg−1) and quercetin 3-O-glucuronide (Q3GND) (235.69 \(\pm \) 1.52 mg kg−1) were found in Csv as major products and neochlorogenic acid (NCA) (52.48 \(\pm \) 1.17 mg kg−1) was the next most abundant product. In addition, the chief products in Csm were Q3GD and Q3GND as a quantity of 49.75 \(\pm \) 3.81 mg kg−1and 107.21 \(\pm \) 9.36 mg kg−1respectively. The total phenolic content of Csv and Csm was found as 1005.80 \(\pm \) 88.17 µgg−1 and 1177.32 \(\pm \) 87.93 µgg−1 respectively.



中文翻译:

HPLC-DAD定量测定香菜和小carp中酚类物质的含量

人类饮食中发现的酚类化合物具有广泛的生物活性。目前的工作描述了酚类化合物的定量分析芫荽大麦(CSV)和芫荽microcarpum通过HPLC-DAD分析(CSM)。通过反相HPLC分析两个品种的甲醇提取物。该方法在线性,精度,准确度,检测限(LOD),定量限(LOQ)和可重复性方面也得到了验证。测定系数(R 2)范围为0.9986-0.9993。LOD和LOQ的范围分别为1.13–1.46至3.42–4.32 mg / kg。还使用Folin–Ciocalteu方法以没食子酸当量(mg g -1干重)表示了两个品种的总酚含量。槲皮素3-β-D葡糖苷(Q3GD)(103.81 \(\ pm \) 4.76 mg kg -1)和槲皮素3-O-葡糖醛酸苷(Q3GND)(235.69 \(\ pm \) 1.52 mg kg -1)为在Csv中作为主要产物被发现,而新绿原酸(NCA)(52.48 ((pm)) 1.17 mg kg -1)是第二丰富的产物。此外,Csm中的主要产品是Q3GD和Q3GND,数量为49.75 \(\ pm \) 3.81 mg kg -1和107.21 \(\ pm \) 9.36 mg kg -1。Csv和Csm的总酚含量分别为1005.80 \(\ pm \) 88.17 µgg -1和1177.32 \(\ pm \) 87.93 µgg -1

更新日期:2021-04-27
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