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ChSch9 is required for infection-related morphogenesis and pathogenicity in Colletotrichum higginsianum
Canadian Journal of Plant Pathology ( IF 2 ) Pub Date : 2021-06-16 , DOI: 10.1080/07060661.2021.1921850
Muhammad Aamir Sohail 1 , Qinfeng Yuan 1 , Yaqin Yan 2 , Junbin Huang 1 , Tom Hsiang 3 , Lu Zheng 1
Affiliation  

Abstract

Colletotrichum higginsianum is an economically important anthracnose pathogen of crucifers that causes losses worldwide. For comprehensive control of anthracnose disease, it is essential to understand the mechanisms of pathogenicity in C. higginsianum. The Ser/Thr kinase Sch9 belonging to the AGC (protein kinase A, G, and C) kinase family, commonly found in eukaryotes, plays a vital role in various cell signalling pathways and development. However, it has not been studied in Colletotrichum species. Three domains, Protein kinase C conserved region 2 (C2), Serine/Threonine protein kinase catalytic (S_TKc) and Extension to Ser/Thr-type protein kinases (S_TK_X), were identified in the C. higginsianum Sch9 (ChSch9) protein. Phylogenetic analysis indicated that ChSch9 is closely related to the AGC kinase family, which is conserved in fungi. Deletion of ChSch9 caused defects in hyphal morphology, growth and conidiation. In addition, deletion of ChSch9 was associated with reduced tolerance to hyperosmotic, cell wall and oxidative stresses in C. higginsianum, and ChSch9 deletion mutants were more tolerant to heat. Furthermore, we found that ChSch9 might be involved in the pyruvate–acetaldehyde fermentation pathway. Importantly, deletion of ChSch9 led to a reduction in appressoria and infective hyphae formation, resulting in defects in virulence. Collectively, the results of this study indicate that ChSch9 is required for infection-related morphogenesis and virulence in C. higginsianum.



中文翻译:

ChSch9 是 Colletotrichum higginsianum 感染相关形态发生和致病性所必需的

摘要

Colletotrichum higginsianum是一种经济上重要的十字花科炭疽病病原体,在全球范围内造成损失。对于炭疽病综合治理,有必要了解致病的机制C. higginsianum。Ser/Thr 激酶 Sch9 属于 AGC(蛋白激酶 A、G 和 C)激酶家族,常见于真核生物中,在各种细胞信号通路和发育中起着至关重要的作用。然而,尚未在炭疽菌属中对其进行研究。在C. higginsianum中鉴定了三个域,蛋白激酶 C 保守区 2 (C2)、丝氨酸/苏氨酸蛋白激酶催化 (S_TKc) 和丝氨酸/苏氨酸型蛋白激酶的延伸 (S_TK_X)Sch9 (ChSch9) 蛋白。系统发育分析表明ChSch9与真菌中保守的AGC激酶家族密切相关。ChSch9 的缺失导致菌丝形态、生长和分生孢子形成缺陷。此外,ChSch9 的缺失与C. higginsianum对高渗、细胞壁和氧化应激的耐受性降低有关,并且ChSch9缺失突变体更耐热。此外,我们发现ChSch9可能参与丙酮酸-乙醛发酵途径。重要的是,删除ChSch9导致抑制菌和感染性菌丝形成减少,导致毒力缺陷。总的来说,这项研究的结果表明ChSch9C. higginsianum感染相关形态发生和毒力所必需的。

更新日期:2021-06-16
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