The idea of using a higher pressure to reduce damage due to freezing has been thoroughly theoretically examined, while there is little experimental evidence on cryopreservation of living systems at a higher hydrostatic pressure. A study was made to assess the viability of HeLa cells at a pressure of 1.0–2.0 kbar, the toxic effects of five classical cryoprotectants under these conditions, and the viability of HeLa cells during slow (conventional) cryopreservation at a pressure of 1.0 or 1.5 kbar. High resistance to higher hydrostatic pressure was experimentally demonstrated for HeLa cells; i.e., 100% cell survival was observed after cell exposure to a pressure of 1.0 kbar for 60 min. The toxic effect of the cryoprotectants increased under pressure in the following order: glycerol < ethylene glycol < 1,2-propanediol = DMSO < DMSO + formamide. Glycerol and ethylene glycol were the least toxic and additionally exerted substantial baroprotective effects. A high cell survival rate of 83 ± 16% was achieved with 10% glycerol used for cryopreservation at a pressure of 1.0 kbar according to fluorescence staining data, but did not exceed the survival rates observed in control normobaric experiments. Freezing was carried out via slow conventional cryopreservation. Different results might be obtained by using vitrification to freeze biological material at a higher pressure.

在理论上已经彻底研究了使用较高的压力来减少由于冷冻造成的损害的想法，而在较高的静水压力下，几乎没有实验证据可以对生物系统进行冷冻保存。进行了一项研究以评估在1.0–2.0 kbar压力下HeLa细胞的活力，在这些条件下五种经典冷冻保护剂的毒性作用以及在1.0或1.5的缓慢（常规）冷冻保存过程中HeLa细胞的活力。 kbar。实验证明了HeLa细胞对较高的静水压力具有较高的抵抗力。也就是说，将细胞暴露于1.0 kbar的压力60分钟后观察到100％的细胞存活率。在压力下，防冻剂的毒性作用按以下顺序增加：甘油<乙二醇<1,2-丙二醇= DMSO < DMSO +甲酰胺。甘油和乙二醇毒性最低，并且还发挥了重要的防压效果。根据荧光染色数据，在1.0 kbar的压力下，将10％的甘油用于冷冻保存，可以达到83±16％的高细胞存活率，但没有超过对照常压实验中观察到的存活率。通过缓慢的常规冷冻保存进行冷冻。通过玻璃化在较高压力下冷冻生物材料可能会获得不同的结果。但没有超过对照常压实验中观察到的生存率。通过缓慢的常规冷冻保存进行冷冻。通过玻璃化在较高压力下冷冻生物材料可能会获得不同的结果。但没有超过对照常压实验中观察到的生存率。通过缓慢的常规冷冻保存进行冷冻。通过玻璃化在较高压力下冷冻生物材料可能会获得不同的结果。