Biophysics Pub Date : 2021-04-27 , DOI: 10.1134/s0006350921010036 M. A. Duk , M. G. Samsonova
The roles that noncoding RNAs play in various cells have been a focus of intense research in recent years. Circular RNAs (circRNAs) are noncoding RNAs that were initially thought to be junk by-products of splicing. Many circRNAs have been found to demonstrate important regulatory functions and, in particular, to act as miRNA sponges. The function of an miRNA sponge was compared with the function of a classic transcription factor (TF)-dependent regulatory loop in terms of efficiency, working rate, and noise characteristics. A circRNA with multiple binding sites for miRNA was found to act more efficiently than a TF and the respective loop worked faster, but only when the binding sites were not fully saturated with miRNA molecules. The noise characteristics of the circRNA loop were significantly worse with an increasing number of binding sites. A circRNA with one binding site was shown to be inefficient as an miRNA sponge. The circRNA-mediated regulation was assumed to provide a specific tool to the cell.
中文翻译:
环状RNA作为miRNA海绵的优点和缺点
近年来,非编码RNA在各种细胞中的作用一直是研究的重点。环状RNA(circRNA)是非编码RNA,最初被认为是剪接的垃圾副产物。已发现许多circRNA表现出重要的调节功能,尤其是充当miRNA海绵。在效率,工作速率和噪声特性方面,将miRNA海绵的功能与经典转录因子(TF)依赖性调节环的功能进行了比较。发现具有多个miRNA结合位点的circRNA比TF更有效率,并且各个环的工作速度更快,但仅当结合位点未完全被miRNA分子饱和时才起作用。随着结合位点数量的增加,circRNA环的噪声特性明显变差。具有一个结合位点的circRNA被证明是无效的miRNA海绵。假定circRNA介导的调控为细胞提供了一种特定的工具。