Rare-cold-inducible 2 (RCI2) genes are involved in plant response to abiotic stresses. In this study, we report the functional role of a Camelina RCI2, CsRCI2D, in plant salt stress response. The localization of CsRCI2D was observed in the plasma membrane and intracellular membranes by confocal analysis in tobacco leaf and western blot analysis in Camelina. The full length CsRCI2D cDNA clone was not able to complement the salt sensitivity of △spmp3 lacking the PMP3 gene. However, a C-terminal tail deleted CsRCI2D cDNA was able to restore the level of salt tolerance to that of WT. CsRCI2D-overexpressing Camelina showed better germination rate and seedling growth. CsRCI2D overexpression decreased Na⁺ accumulation in both roots and shoots but increased K⁺ accumulation in the shoots under salt stress. Furthermore, CsRCI2D-overexpressing Camelina displayed lower H₂O₂ and malondialdehyde (MDA) upon salt stress. Under normal growth condition, CsRCI2D-overexpressing Camelina showed higher transcript levels of all antioxidant genes (CsCuSODs, CsMnSOD1, CsFeSODs, CsCATs, CsAPX1, and CsGR), whereas salt stress significantly induced all antioxidant genes in WT. These results indicate that salt-upregulated CsRCI2D plays a positive role in Camelina seed germination and seedling growth under salt stress by maintenance of ion homeostasis and modulating the expression of antioxidant-related genes.

罕见冷诱导型2（RCI2）基因参与植物对非生物胁迫的反应。在这项研究中，我们报告的职能作用亚麻RCI2，CsRCI2D，在植物盐胁迫的响应。通过在烟叶和免疫印迹分析共焦分析在质膜和细胞内膜观察CsRCI2D的定位亚麻荠。全长CsRCI2D cDNA克隆不能补充缺少PMP3基因的△spmp3的盐敏感性。但是，C末端尾部缺失的CsRCI2D cDNA能够将耐盐性水平恢复为野生型。CsRCI2D -overexpressing亚麻表现出较好的发芽率和幼苗生长。在盐胁迫下，CsRCI2D过表达减少了根和茎中的Na ⁺积累，但增加了芽中的K ⁺积累。此外，CsRCI2D -overexpressing荠显示下ħ ₂ ö ₂和丙二醛（MDA）时盐胁迫。在正常的生长条件，CsRCI2D -overexpressing亚麻显示所有抗氧化基因（更高的转录水平CsCuSODs，CsMnSOD1，CsFeSODs，CsCATs，CsAPX1，并CsGR），而盐胁迫显着诱导野生型中的所有抗氧化基因。这些结果表明，盐上调CsRCI2D起着积极的作用亚麻种子萌发，并通过维护离子稳态的幼苗盐胁迫下的生长和调制的抗氧化剂相关的基因的表达。