Objective

Spinal cord injury (SCI) is a severe traumatic disease in the central nervous system, and can result in neuronal injury. Altered miRNA expression is identified to be involved in the pathogenesis of SCI.

Design

This study investigated the clinical value of miR-142-5p in SCI patients, and explored its functional role in the regulation of inflammatory.

Setting

The First Affiliated Hospital of Soochow University.

Participants

Ninety-eight patients with acute spinal trauma.

Interventions

All patients were recruited, and divided into complete SCI group, incomplete SCI group and normal nerve function group.

Outcome Measures

Real-time quantitative PCR (qRT-PCR) was used to detect the expression levels of miR-142-5p. CCK-8 and flow cytometry assay were performed to evaluate the cell viability and apoptosis. ELISA assay was applied to estimate the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).

Results

Serum miR-142-5p level was significantly increased in SCI patients, especially the complete SCI cases. ROC curve analysis suggested miR-142-5p could distinguish SCI patients from normal nerve function patients and was associated with the severity of SCI. A positive association was detected between miR-142-5p and serum levels of IL-6, TNF-α in SCI patients. Downregulation of miR-142-5p significantly reduced the protein levels of both IL-6 and TNF-α in LPS treated PC12 cells, and weakened LPS induced cell apoptosis.

Conclusion

MiR-142-5p is a potential biomarker for the occurrence of SCI in acute spinal trauma patients. Downregulation of miR-142-5p plays an anti-inflammatory effect for SCI patients.

中文翻译：

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miR-142-5p在脊柱外伤致脊髓损伤中的临床意义及其在炎症调控中的功能作用

客观的

脊髓损伤（SCI）是中枢神经系统的严重创伤性疾病，可导致神经元损伤。改变的 miRNA 表达被鉴定为参与 SCI 的发病机制。

设计

本研究调查了 miR-142-5p 在 SCI 患者中的临床价值，并探讨了其在炎症调节中的功能作用。

环境

苏州大学附属第一医院．

参加者

98 名急性脊柱外伤患者。

干预措施

纳入所有患者，分为完全性SCI组、不完全性SCI组和神经功能正常组。

结果措施

使用实时定量PCR（qRT-PCR）检测miR-142-5p的表达水平。进行CCK-8和流式细胞术测定以评估细胞活力和细胞凋亡。应用 ELISA 测定来估计白细胞介素 6 (IL-6) 和肿瘤坏死因子-α (TNF-α) 的水平。

结果

SCI 患者血清 miR-142-5p 水平显着升高，尤其是完全 SCI 病例。ROC曲线分析表明miR-142-5p可以区分SCI患者和神经功能正常的患者，并且与SCI的严重程度相关。在 SCI 患者中检测到 miR-142-5p 与血清 IL-6、TNF-α 水平呈正相关。下调 miR-142-5p 可显着降低 LPS 处理的 PC12 细胞中 IL-6 和 TNF-α 的蛋白水平，并减弱 LPS 诱导的细胞凋亡。

结论

MiR-142-5p 是急性脊柱外伤患者发生 SCI 的潜在生物标志物。miR-142-5p 的下调对 SCI 患者具有抗炎作用。