In this work, novel types of nitrogen-doped carbon dots (N-CDs) were prepared from citric acid and glycine (GLY) as precursors through a simple pyrolysis method. The GLY-CDs showed strong fluorescence with a fluorescence quantum yield as high as 33.34% and good water solubility. The fluorescence of GLY-CDs could be selectively quenched by iron(III) ion (Fe³⁺) resulting in the non-fluorescent complex. Due to the high affinity of Fe³⁺ to adenosine-5′-triphosphate (ATP), the fluorescence of the GLY-CDs in GLY-CDs-Fe³⁺ could be recovered by ATP. Thereby, quantitatively fluorescent turn-on detection of ATP could be achieved. The fluorescence recovery ratio was linearly proportional to the concentration of ATP with a detection limit as low as 15.0 nM, indicating the CDs have high sensitivity. The GLY-CDs were successfully employed in the detection of ATP in serum and cell lysates.

中文翻译：

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基于氮掺杂碳点的血清和裂解物中 ATP 的超灵敏检测

在这项工作中，以柠檬酸和甘氨酸 (GLY) 作为前驱体，通过简单的热解方法制备了新型氮掺杂碳点 (N-CD)。GLY-CDs 具有强荧光，荧光量子产率高达 33.34%，水溶性良好。GLY-CDs 的荧光可以被铁 (III) 离子 (Fe ³⁺ )选择性淬灭，从而形成非荧光复合物。由于 Fe ³⁺对 5'-三磷酸腺苷 (ATP)的高亲和力，GLY-CDs-Fe ³⁺中 GLY-CDs 的荧光可以被ATP回收。因此，可以实现ATP的定量荧光开启检测。荧光回收率与 ATP 浓度成线性正比，检测限低至 15.0 nM，表明 CD 具有高灵敏度。GLY-CD 已成功用于检测血清和细胞裂解物中的 ATP。