Animal feed is typically plant-based and can contain pesticide residues. Methods for testing food and feed samples, such as the Quick Easy Cheap Effective Rugged Safe (QuEChERS) method or the Swedish Ethyl Acetate (SweEt) method, successfully extract many pesticide residues. However, nonpolar pesticides, such as organochlorine pesticides, show poor recovery when extracted from lipid-rich samples. The previously developed water-acetonitrile-heptane-solid-phase-extraction (WAHSPE) method shows better recoveries for the nonpolar pesticides but requires two injections per sample and per instrument. Here, we present a modified version of the WAHSPE method for pesticides in fish feed using one injection per sample and per instrument. Of the 184 pesticides tested, 179 met the European Union Legislation’s validation criteria at a spike level of 50 μg/kg, showing recoveries between 70 and 120% and a relative standard deviation (RSD) below 20%. Organochlorine pesticides accounted for 14 of the tested compounds.

中文翻译：

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新型通用提取方法与气相色谱-液相色谱-质谱联用对高脂鱼饲料中184种农药进行多残留分析

动物饲料通常以植物为基础，并且可能含有农药残留。食品和饲料样品的测试方法，例如快速简便便宜有效的坚固耐用型（QuEChERS）方法或瑞典乙酸乙酯（SweEt）方法，成功地提取了许多农药残留。但是，当从富含脂质的样品中提取时，非极性农药（例如有机氯农药）的回收率很低。先前开发的水-乙腈-庚烷-固相萃取（WAHSPE）方法对非极性农药的回收率更高，但每个样品和每个仪器需要进样两次。在这里，我们介绍了一种用于鱼类饲料中农药的WAHSPE方法的改进版本，每个样品和每种仪器一次注射。在测试的184种农药中，有179种达到了欧盟法规的验证标准，峰值水平为50μg/ kg，回收率在70％至120％之间，相对标准偏差（RSD）低于20％。有机氯农药占测试化合物的14种。