In this study, we aimed to observe the effects of Loofah cylindrica (LC) extract on learning and memory ability, brain tissue morphology, and immune function of aging mice. Kunming mice were selected and randomly divided into a control group, a positive control group, an aging group, and three dose groups. Three dose groups were administered 187.5, 375, and 750 mg/kg of LC extract, respectively. Except for the control group, the mice in any other group were continuously subcutaneously injected d -galactose on the back and neck. Platform tests and Morris water maze (MWM) were adopted to test the learning and memory ability of each group. The brain index, thymus index, and spleen index of each group were determined by the organ-to-body ratio method. The enzyme-linked immunosorbent assay was performed to measure the concentration of cytokines interleukin 2 and interferon gamma and the proliferation activity of T lymphocytes in mouse serum. In addition, the hematoxylin and eosin staining was employed to observe the morphological changes in mouse brain tissues of each group. The results show that the aging group made more errors in the platform test, had longer escape latency, shorter swimming time in the platform quadrant, and fewer platform crossings in the MWM; much fewer brain tissue cells; and smaller brain index, thymus index, and spleen index. The LC extracts (375 and 750 mg/kg) can significantly antagonize the changes in the above indices. It can be concluded that LC extract can improve the learning and memory of aging mice, enhance their immune activity, and delay the aging process.

中文翻译：

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丝瓜络提取物对衰老小鼠学习记忆能力，脑组织形态和免疫功能的影响

在这项研究中，我们旨在观察丝瓜络（LC）提取物对衰老小鼠的学习和记忆能力，脑组织形态以及免疫功能的影响。选择昆明小鼠并将其随机分为对照组，阳性对照组，衰老组和三个剂量组。三个剂量组分别给予187.5、375和750 mg / kg的LC提取物。除对照组外，任何其他组的小鼠在背部和颈部连续皮下注射d-半乳糖。采用平台测试和莫里斯水迷宫（MWM）测试每组的学习和记忆能力。每组的脑指数，胸腺指数和脾脏指数通过器官与身体的比率方法确定。进行酶联免疫吸附测定以测量小鼠血清中细胞因子白介素2和干扰素γ的浓度以及T淋巴细胞的增殖活性。此外，使用苏木精和曙红染色观察各组小鼠脑组织的形态变化。结果表明，老化组在平台测试中犯了更多的错误，逃脱潜伏期更长，平台象限中的游泳时间更短，MWM中的平台穿越次数更少。脑组织细胞少得多；以及较小的大脑指数，胸腺指数和脾脏指数。LC提取物（375和750 mg / kg）可以明显拮抗上述指标的变化。可以得出结论，LC提取物可以改善衰老小鼠的学习和记忆能力，增强其免疫活性，并延缓衰老过程。