The Mayan cichlid (Cichlasoma urophthalmus), is a freshwater fish of the Cichlidae family, native to the south east of Mexico, it has potential for extensive cultivation due to its excellent meat quality and local demand. However, the nutrigenomic aspects and molecular expression of genes involved in its digestion that could contribute to its growth during the larval stage are unknown. Some enzymes such as Pepsin (PEP), pancreatic such as trypsin (TRY), Chymotrypsin [CHY] and intestinal enzymes such as Leucine aminopeptidase [ANPEP], function during larval ontogenesis and activity patterns of the key factors involved in the digestive system. For this study, 30–50 larvae were collected, which were processed with mRNA extraction techniques and cDNA synthesis by RT-PCR using qPCR oligos designed from Oreochromis niloticus, measuring molecular expression during the initial ontogeny of C. urophthalmus. The results obtained show the molecular expression of these enzymes occurs through epigenetic factors such as changes from endogenous to exogenous feeding, presenting the activation of genes for the formation of organs of the digestive tract that allow the secretion of specific enzymes for processing and digestion of food, that can activate these genes to increase the digestion efficiency and the optimal use of nutrients.

中文翻译：

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玛雅慈鲷（Cichlasoma urophthalmus）个体发育过程中消化酶的分子表达

玛雅慈鲷（Cichlasoma urophthalmus）是慈鲷科的一种淡水鱼，原产于墨西哥东南部，由于其优良的肉质和当地需求，具有广泛养殖的潜力。然而，在其幼虫阶段可能有助于其生长的消化所涉及的基因的营养基因组方面和分子表达尚不清楚。一些酶，如胃蛋白酶 (PEP)、胰蛋白酶 (TRY)、胰凝乳蛋白酶 [CHY] 和肠道酶 (如亮氨酸氨肽酶 [ANPEP])，在幼虫个体发育过程中发挥作用，是参与消化系统的关键因素的活动模式。在本研究中，收集了 30-50 只幼虫，使用 mRNA 提取技术和 RT-PCR 合成 cDNA，使用设计的 qPCR 寡核苷酸进行处理。Oreochromis niloticus，在C. urophthalmus的初始个体发育过程中测量分子表达。获得的结果表明，这些酶的分子表达是通过表观遗传因素发生的，例如从内源性到外源性喂养的变化，呈现出用于形成消化道器官的基因的激活，这些器官允许分泌用于加工和消化食物的特定酶，可以激活这些基因以提高消化效率和营养物质的最佳利用。