A survey was carried out in 19 districts to investigate the prevalence and distribution of sweetpotato virus disease (SPVD) and its implication on the sustainability of clean seed system in Malawi. A total of 166 leaf samples were collected and tested for the presence of 8 viruses using nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). SPVD foliar symptoms were observed in 68.42% of the surveyed districts. There were significant variations in disease incidence and severity (p < 0.001) among districts, with the highest incidence in Mulanje (28.34%). Average SPVD severity score was 3.05. NCM-ELISA detected sweet potato feathery mottle virus (SPFMV, 30.54%), sweet potato mild mottle virus (SPMMV, 31.14%), sweet potato mild speckling virus (SPMSV, 16.17%), sweet potato C-6 virus (SPC6V, 13.77%), sweet potato chlorotic stunt virus (SPCSV, 22.16%), sweet potato collusive virus (SPCV, 30.54%), sweet potato virus G (SPVG, 11.38%), cucumber mosaic virus (CMV, 7.78%) either in single or mixed infections. Data from this study indicate a significant SPVD occurrence in the country, and the consequence implications towards national sweetpotato seed system.

在19个地区进行了一项调查，以调查甘薯病毒病（SPVD）的流行和分布及其对马拉维清洁种子系统可持续性的影响。总共收集了166个叶样品，并使用硝酸纤维素膜酶联免疫吸附测定（NCM-ELISA）测试了8种病毒的存在。在被调查的地区中，有68.42％的人观察到SPVD的叶状症状。各地区的疾病发病率和严重程度差异显着（p <0.001），其中穆兰杰的发病率最高（28.34％）。SPVD的平均严重性评分为3.05。NCM-ELISA检测到地瓜羽毛状斑驳病毒（SPFMV，30.54％），地瓜轻度斑驳病毒（SPMMV，31.14％），地瓜轻度斑点病毒（SPMSV，16.17％），地瓜C-6病毒（SPC6V，13.77） ％），单一或混合感染中的红薯褪绿特技病毒（SPCSV，22.16％），红薯串谋病毒（SPCV，30.54％），红薯病毒G（SPVG，11.38％），黄瓜花叶病毒（CMV，7.78％）。这项研究的数据表明该国SPVD发生率很高，其后果对国家甘薯种子系统产生了影响。