Abstract

Purpose

Quinoa is an annual plant that grows well in high altitude regions with high radiation and ultraviolet intensity. It has known that high-dose radiation damages living organisms, but low-dose radiation also has a beneficial effect. Therefore, the purpose of this study is to investigate the hormesis effect of gamma-ray on quinoa by growth analysis and hyperspectral imaging.

Materials and methods

Quinoa seeds were irradiated at 50, 100, and 200 Gy emitted by ⁶⁰CO. Subsequently, the seeds were germinated and transplanted into pots, then conducted growth analysis and physiological evaluation every week, and hyperspectral imaging. Photosynthetic ability was measured at 35 days after transplanting (DAT), and the plants for each dose were divided into aerial and underground parts for biomass evaluation at 91 DAT. Various vegetation indices were estimated from 14 to 35 DAT by hyperspectral analysis, and the specific bands were extracted based on the PLS model using plant height, SPAD value, and chlorophyll fluorescence parameters.

Results

We found that plant height and biomass were increased in quinoa plants treated with a low dose (50 Gy) as compared to control. Chlorophyll content and chlorophyll fluorescence were not different between doses at the early growth stage, but as growth progressed, the plant irradiated at 200 Gy began to be lower. The photosynthetic ability of the quinoa plant treated at 50 Gy was greater than other plants at 35 DAT. The vegetation indices related to the pigment status also were higher in the plants treated by irradiation at 50 Gy than the plants grown in other doses treatment units at the beginning of the growth. Using the PLS model we collected sensitive band wavelengths from hyperspectral image analysis. Among the collected bands, eight bands closely related to plant height, nine bands to chlorophyll content, and ten bands to chlorophyll fluorescence were identified.

Conclusion

Our results showed that the growth and physiological parameters of quinoa treated by low dose gamma irradiation to seeds were greater than that of control as well as the plant with higher doses. These findings confirm that the positive changes in the characteristics of quinoa with low dose radiation indicated that hormesis occurs at 50 Gy radiation.

中文翻译：

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伽马辐射对藜（Chenopodium quinoa）生长的刺激作用

摘要

目的

藜麦是一年生植物，在高辐射和紫外线强度高的地区生长良好。众所周知，高剂量辐射会损害生物体，但低剂量辐射也有好处。因此，本研究的目的是通过生长分析和高光谱成像研究伽马射线对藜麦的刺激作用。

材料和方法

藜麦种子分别用⁶⁰ CO辐射 50、100 和 200 Gy 。随后，种子发芽并移植到盆中，然后每周进行生长分析和生理评估，并进行高光谱成像。在移栽后 35 天（DAT）测量光合能力，并将每个剂量的植物分为地上和地下部分，在 91 DAT 进行生物量评估。通过高光谱分析估计了14至35 DAT的各种植被指数，并使用植物高度、SPAD值和叶绿素荧光参数基于PLS模型提取特定波段。

结果

我们发现，与对照相比，用低剂量 (50 Gy) 处理的藜麦植物的株高和生物量增加。叶绿素含量和叶绿素荧光在生长早期各剂量之间没有差异，但随着生长的进行，200 Gy 辐照的植物开始降低。以 50 Gy 处理的藜麦植物的光合能力在 35 DAT 时高于其他植物。在生长开始时，通过 50 Gy 辐射处理的植物中与色素状态相关的植被指数也高于在其他剂量处理单元中生长的植物。使用 PLS 模型，我们从高光谱图像分析中收集了敏感波段波长。采集到的谱带中，与株高密切相关的谱带有8条，与叶绿素含量有关的谱带有9条，

结论

我们的结果表明，低剂量γ辐照种子处理藜麦的生长和生理参数高于对照和高剂量植物。这些发现证实，低剂量辐射下藜麦特性的积极变化表明，在 50 Gy 辐射下会发生兴奋作用。