This study represents synthesis, characterization, screening of antibiofilm efficacy, and cytotoxicity of iridium bis-terpyridine complexes. The complexes were characterized by NMR, MS, FTIR, UV/Visible, and fluorescence spectroscopies. The efficacy of biofilm inhibition and eradication of iridium complexes was evaluated using a crystal violet assay test and verified by fluorescence microscopy. Cytotoxicity and apoptosis analysis of iridium complexes were determined in this study. The results of our study revealed that three iridium complexes had the potential to inhibit biofilm formation and moderate the ability to destroy pre-formed biofilm of S. aureus ATCC 29,213. 250 µM concentration of synthesized complexes showed the highest antibiofilm activity (75% for Ir1, 90% for Ir2, and 71% for Ir3). The significant inhibition obtained at 6.25 µM concentration of Ir2 and Ir3 revealed the potential of our samples. Also, Ir1 and Ir2 complexes had a good capacity to destroy pre-formed biofilm. The results clearly showed that iridium complexes have cytotoxic activity towards colon cancer (Caco-2) and liver cancer (HepG2) cell lines without affecting non-cancerous cells (HEK293) at applied doses. Moreover, tested compounds induced apoptosis in these cancer cells. All of these results showed that iridium complexes had possessed the ability to inhibit or destroy pre-formed biofilm and could be developed as an effective agent against bacterial biofilms. Moreover, these pure substances may have valuable anti-cancer activity and it should be confirmed with further studies for therapeutic effects.

这项研究代表了铱双三联吡啶复合物的合成、表征、筛选抗生物膜功效和细胞毒性。通过 NMR、MS、FTIR、UV/可见光和荧光光谱对配合物进行表征。使用结晶紫测定试验评估了生物膜抑制和消除铱复合物的功效，并通过荧光显微镜进行了验证。本研究确定了铱复合物的细胞毒性和细胞凋亡分析。我们的研究结果表明，三种铱配合物具有抑制生物膜形成的潜力，并调节破坏金黄色葡萄球菌预先形成的生物膜的能力ATCC 29,213。250 µM 浓度的合成复合物显示出最高的抗生物膜活性（Ir1 为 75%，Ir2 为 90%，Ir3 为 71%）。在 6.25 µM 浓度的 Ir2 和 Ir3 下获得的显着抑制揭示了我们样品的潜力。此外，Ir1 和 Ir2 复合物具有良好的破坏预先形成的生物膜的能力。结果清楚地表明，铱复合物对结肠癌 (Caco-2) 和肝癌 (HepG2) 细胞系具有细胞毒活性，而在应用剂量下不影响非癌细胞 (HEK293)。此外，测试的化合物在这些癌细胞中诱导细胞凋亡。所有这些结果表明，铱络合物具有抑制或破坏预先形成的生物膜的能力，可以作为一种有效的抗细菌生物膜剂。而且，