Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but are impeded by their low viability and efficiency after grafting in vivo. Nitric oxide (NO) is beneficial in promoting stem cell bioactivity, but whether it can function effectively in vivo is still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs and NO (3D-ADSCs/NO) to evaluate its biological efficacy in promoting severe burn wound healing. The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting. Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs). Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition. Notably, immunohistochemistry of CD31 suggested an increase in neovascularization, supported by the upregulation of vascular endothelial growth factor (VEGF) mRNA in ADSCs in the 3D biosystem. These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway. This scaffold may be considered a promising strategy for healing severe burns.

中文翻译：

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整体 ADSCs-NO 水凝胶支架的 3D 生物打印促进严重烧伤伤口愈合

严重烧伤难以治愈，并导致全世界大量死亡。脂肪来源的间充质干细胞 (ADSCs) 已成为一种有前途的全层烧伤愈合治疗方法，但由于其在体内移植后的低活力和效率而受到阻碍。一氧化氮 (NO) 有利于促进干细胞的生物活性，但它是否能在体内有效发挥作用仍然很大程度上是未知的。在这项研究中，我们生物打印了一种负载 ADSCs 和 NO (3D-ADSCs/NO) 的高效生物支架，以评估其促进严重烧伤伤口愈合的生物功效。整体 3D-ADSCs/NO 水凝胶支架是通过 3D 生物打印构建的。我们的研究结果表明，3D-ADSCs/NO 可以增强人脐静脉内皮细胞 (HUVECs) 的迁移和血管生成。小鼠烧伤伤口愈合实验表明，3D-ADSCs/NO 通过促进更快的上皮化和胶原沉积来加速伤口愈合。值得注意的是，CD31 的免疫组织化学表明新血管形成增加，这得到了 3D 生物系统中 ADSC 中血管内皮生长因子 (VEGF) mRNA 的上调的支持。这些发现表明，3D-ADSC/NO 水凝胶支架可通过 VEGF 信号通路增加新血管形成来促进严重烧伤创面的愈合。这种支架可能被认为是治愈严重烧伤的有前途的策略。由 3D 生物系统中 ADSC 中血管内皮生长因子 (VEGF) mRNA 的上调支持。这些发现表明，3D-ADSC/NO 水凝胶支架可通过 VEGF 信号通路增加新血管形成来促进严重烧伤创面的愈合。这种支架可能被认为是治愈严重烧伤的有前途的策略。由 3D 生物系统中 ADSC 中血管内皮生长因子 (VEGF) mRNA 的上调支持。这些发现表明，3D-ADSC/NO 水凝胶支架可通过 VEGF 信号通路增加新血管形成来促进严重烧伤创面的愈合。这种支架可能被认为是治愈严重烧伤的有前途的策略。