An in vivo micrografting method was developed to obtain Passiflora latent virus (PLV)-free passion fruit from infected plants. The scion length required to eliminate PLV was ≤ 2 mm. The method required no aseptic handling and the procedure was relatively simple, and resulted in more than 10 micrografts in one hour. Moreover, no special equipment or procedures were necessary, allowing it to be conducted at an individual farm level. Rapid growth of the scion after grafting was also observed due to the use of fully established seedlings as rootstock. Leaf samples for analysis of PLV infection could therefore be obtained about two months after grafting with fruit harvest possible about four months later. In vivo micrografting was conducted with a scion length of 0.5–1 mm from September to November to determine the optimal air temperature conditions. The graft success rate increased from 18% to 58% with a decrease in the average air temperature from September (28.6°C) to November (23.3°C), although there was no significant difference in PLV-free rates between months (73% to 80%). Accordingly, in vivo micrografting of passion fruit is not recommended under high air temperature conditions. The effect of scion shoot storage conditions was also examined, revealing that in vivo micrografting using shoots stored for one day could be performed without difficulty, whereas the rate of graft success and PLV-free rate were close to those obtained using shoots selected less than 30 min before. These findings suggest that with this method, virus-free plants can be obtained using PLV-infected shoots selected in the field one day earlier. In conclusion, our in vivo micrografting technique is useful to eliminate PLV from infected plants; it is simple and leads to rapid growth of the scion after grafting.

开发了一种体内显微移植方法，以从受感染的植物中获得无西番莲潜伏病毒 (PLV) 的百香果。消除 PLV 所需的接穗长度≤ 2 mm。该方法不需要无菌操作，程序相对简单，1小时内产生了10多个微移植物。此外，不需要特殊设备或程序，可以在单个农场级别进行。由于使用完全成熟的幼苗作为砧木，还观察到接穗在嫁接后快速生长。因此，可在嫁接后约两个月获得用于分析 PLV 感染的叶样本，约四个月后收获果实。体内从 9 月到 11 月，以 0.5-1 毫米的接穗长度进行微嫁接，以确定最佳气温条件。随着 9 月 (28.6°C) 至 11 月 (23.3°C) 的平均气温降低，移植成功率从 18% 增加到 58%，尽管月间无 PLV 率 (73%)到 80%）。因此，不建议在高温条件下对百香果进行体内显微移植。还检查了接穗存储条件的影响，表明在体内使用储存一天的枝条进行微嫁接可以毫无困难地进行，而移植成功率和无 PLV 率与使用不到 30 分钟前选择的枝条获得的接近。这些发现表明，通过这种方法，可以使用一天前在田间选择的受 PLV 感染的枝条获得无病毒植物。总之，我们的体内显微移植技术可用于消除受感染植物中的 PLV；操作简单，嫁接后接穗生长迅速。