Lipid-coated microbubbles (MBs) with an indocyanine green (ICG) derivative were fabricated for ultrasound and near-infrared (NIR) fluorescence dual imaging. We characterized the NIR-fluorescence intensity, stability and viscoelastic properties of the encapsulating lipid shell, focusing on the influence of the ICG derivative and lipid compositions. In terms of the NIR fluorescence intensity, the fluorescence intensity of the MBs (with the ICG derivative) was significantly affected by the lipid composition of the MB shell. Regarding the contrast agent used for ultrasound imaging, the stability of the MBs and viscoelastic properties of shell also depended on the lipid compositions, while the incorporation of the ICG derivative into the MB shells had a negligible effect. The performance of this contrast agent for ultrasound and NIR fluorescence dual-imaging exhibited a significant trade-off relationship for the lipid composition.

带有吲哚菁绿 (ICG) 衍生物的脂质涂层微泡 (MB) 被制造用于超声和近红外 (NIR) 荧光双重成像。我们表征了封装脂质壳的 NIR 荧光强度、稳定性和粘弹性，重点关注 ICG 衍生物和脂质成分的影响。在 NIR 荧光强度方面，MBs（带有 ICG 衍生物）的荧光强度受 MB 壳的脂质组成的显着影响。关于用于超声成像的造影剂，MB 的稳定性和壳的粘弹性也取决于脂质成分，而将 ICG 衍生物掺入 MB 壳的影响可以忽略不计。