Background

Wound healing disorders characterised by impaired or delayed reepithelialisation are a serious medical problem. In the present study, we show that gold-based blood serum therapy is a suitable therapeutic approach and shows a supportive effect in wound closure of human corneal epithelial cells (HCE) in primary in vitro experiments.

Methods

For this purpose, blood from healthy individuals was incubated without (S.Ctrl) or with gold-microparticles (S.Therapy) for 24 h. Prior to human epithelial cell stimulation (HCE), the gold particles were removed and the serum was diluted in DMEM (10 % or 30 %). Both groups of serum were compared after injury. HCE were cultured and injured (corneal in vitro wound model) and then stimulated with S.Ctrl or S.Therapy.

Results

Treatment with serum from a gold-based serum therapy (S.Therapy) shows a supportive effect on wound healing in HCE cells in vitro. In addition, gold therapy supports the secretion of important cytokines normally associated with ocular surface wound healing (IL-1β, IL-6, TNF-α and TGF-β) in HCE cells.

Conclusions

Therapy with gold-based blood serum significantly promotes the secretion and expression of cytokines and growth factors in HCE cells in vitro.

Further preclinical experiments are necessary to demonstrate the influence of this therapy on HCE cells for possible clinical application on the human ocular surface and to prove its function also in poorly healing corneal lesions.

中文翻译：

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金基血清处理促进角膜上皮细胞缺损创面闭合的初步体外实验

背景

以受损或延迟的上皮再生为特征的伤口愈合障碍是严重的医学问题。在本研究中，我们表明基于金的血清疗法是一种合适的治疗方法，并且在初步体外实验中显示出对人角膜上皮细胞 (HCE) 伤口闭合的支持作用。

方法

为此，健康个体的血液在没有（S.Ctrl）或金微粒（S.Therapy）的情况下孵育 24 小时。在人上皮细胞刺激 (HCE) 之前，去除金颗粒并将血清稀释在 DMEM（10% 或 30%）中。伤后比较两组血清。HCE 被培养和损伤（角膜体外伤口模型），然后用 S.Ctrl 或 S.Therapy 刺激。

结果

用基于金的血清疗法 (S.Therapy) 的血清治疗显示出对体外HCE 细胞伤口愈合的支持作用。此外，金疗法支持 HCE 细胞中通常与眼表伤口愈合相关的重要细胞因子（IL-1β、IL-6、TNF-α 和 TGF-β）的分泌。

结论

金基血清治疗显着促进体外HCE细胞中细胞因子和生长因子的分泌和表达。

需要进一步的临床前实验来证明这种疗法对 HCE 细胞的影响，以便可能在人眼表面临床应用，并证明其在愈合不良的角膜病变中的功能。