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Separation of Storage Proteins (7S and 11S) from Soybean Seed, Meals and Protein Isolate Using an Optimized Method Via Comparison of Yield and Purity
The Protein Journal ( IF 3 ) Pub Date : 2021-04-24 , DOI: 10.1007/s10930-021-09990-9
Jalal Ud Din 1 , Abid Sarwar 1 , You Li 1 , Tariq Aziz 1 , Fida Hussain 2 , Syed Muhammad Mukarram Shah 2 , Guofu Yi 1 , Xinqi Liu 1
Affiliation  

The primary purpose of this study was to extract β-conglycinin (7S) and glycinin (11S) from soybean seed, soybean meals and soybean protein isolate and compare their yield and purity. The previous methods were modified for the extraction and isolation of 7S and 11S globulins. The adjustment mainly included sample to solution ratio of 1:10 (previously 1:15). Comparing the yield of 11S fraction in Tris–HCl and water as extractable solutions, it was almost doubled in soybean seed (16.97% to 32.41%) with purity from 96 to 98% respectively. In case of soybean meal, samples yield increased from 45.46 to 61.86% with purity from 94 to 98%. On contrary, 7S yield was significantly improved in soybean protein isolate sample from 30.33 to 53.81% along with no contamination in its purity while soybean seed and soybean meal samples had less increase in both yield and purity in Tris–HCl and water as extractable solutions. Results of this study will bring new insights into soybean 7S and 11S separation and purification techniques as well as pave the way for their application in food industry.



中文翻译:

通过比较产量和纯度,使用优化方法从大豆种子、豆粕和蛋白质分离物中分离储存蛋白质(7S 和 11S)

本研究的主要目的是从大豆种子、豆粕和大豆分离蛋白中提取 β-伴大豆球蛋白 (7S) 和大豆球蛋白 (11S),并比较它们的产量和纯度。对之前的方法进行了修改,用于 7S 和 11S 球蛋白的提取和分离。调整主要包括样品与溶液的比例为 1:10(以前为 1:15)。比较 11S 馏分在 Tris-HCl 和水中作为可提取溶液的产率,大豆种子中的 11S 馏分几乎翻了一番(16.97% 至 32.41%),纯度分别为 96% 至 98%。在豆粕的情况下,样品产量从 45.46% 增加到 61.86%,纯度从 94% 增加到 98%。相反,大豆分离蛋白样品的 7S 产率从 30.33 显着提高到 53。81% 且纯度没有污染,而大豆种子和豆粕样品在 Tris-HCl 和水中作为可提取溶液的产量和纯度增加较少。该研究结果将为大豆 7S 和 11S 分离纯化技术带来新的见解,并为其在食品工业中的应用铺平道路。

更新日期:2021-04-24
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