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Optimization of dietary pyridoxine improved growth performance, hematological indices, antioxidant capacity, intestinal enzyme activity, non-specific immune response, and liver pyridoxine concentration of fingerling major carp Catla catla (Hamilton)
Aquaculture ( IF 4.5 ) Pub Date : 2021-04-24 , DOI: 10.1016/j.aquaculture.2021.736815
Younis Mohd Khan , Mukhtar A. Khan

The current research work was undertaken to evaluate the requirement of fingerling Catla catla (3.7 ± 0.5 g) for dietary pyridoxine. Triplicate groups of fingerling were fed casein-gelatin based iso-nitrogenous and isocaloric diets (350 g/kg Crude Protein; 16.72 Kj/g Gross Energy) containing eight different levels of pyridoxine (0, 0.5, 1, 2, 4, 6, 8 and 10 mg/kg diet) to satiation for 16 weeks. All the growth parameters were positively affected by increasing concentrations of dietary pyridoxine up to 4 mg/kg diet. Erythrocyte (RBC), hematocrit (Ht), hemoglobin (Hb), alanine (ALT) and aspartate aminotransferase (AST) activities were significantly (P < 0.05) affected and increased up to 4 mg pyridoxine per kg diet. Thiobarbituric acid reactive substances (TBARS) values were found to be highest in fish fed basal diet which decreased with the increase of dietary pyridoxine up to 4 mg/kg whereas, liver superoxide dismutase (SOD) and catalase (CAT) activities increased with incremental levels of dietary pyridoxine up to 4 mg/kg diet. Intestinal protease, amylase and lipase activities significantly (P < 0.05) increased as the amounts of dietary pyridoxine increased up to 4 mg/kg and then leveled off. Fish fed 4 mg/kg pyridoxine reflected significantly higher (P < 0.05) lysozyme, tissue serum protein and alkaline phosphatase activities than fish fed the pyridoxine-deficient diets. However, liver saturation was achieved in fish fed 8 mg pyridoxine per kg diet. Broken-line regression analysis of growth parameters, RNA/DNA ratio, SOD, protease, lysozyme and the liver pyridoxine concentration data against varying levels of dietary pyridoxine indicated the requirement in the range of 3.20–6.87 mg/kg.



中文翻译:

日粮吡ido醇的优化改善了鱼种主要鲤鱼Catla catla(Hamilton)的生长性能,血液学指标,抗氧化能力,肠道酶活性,非特异性免疫反应和肝脏吡ido醇浓度

当前的研究工作用于评估膳食吡ido醇对鱼种Catla catla(3.7±0.5 g)的需求。一式三份的鱼种进食酪蛋白-明胶等氮和等热量饮食(350 g / kg粗蛋白; 16.72 Kj / g总能量),其中包含八种不同水平的吡ido醇(0、0.5、1、2、4、6、6) 8和10 mg / kg的饮食)至饱食16周。饮食中吡ido醇的浓度增加至4 mg / kg饮食对所有生长参数均产生积极影响。红细胞(RBC),血细胞比容(Ht),血红蛋白(Hb),丙氨酸(ALT)和天冬氨酸转氨酶(AST)的活性显着(P (<0.05)会影响并增加至每公斤饮食4毫克吡ido醇。基础饲料中鱼的硫代巴比妥酸反应性物质(TBARS)值最高,随饲料中吡ido醇含量增加至4 mg / kg的增加而降低,而肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性则随着水平的增加而增加饮食中的吡ido醇最高至4 mg / kg饮食。 随着饮食中吡ido醇含量的增加,肠道蛋白酶,淀粉酶和脂肪酶的活性显着增加(P <0.05),直至达到4 mg / kg,然后趋于平稳。饲喂4 mg / kg吡ido醇的鱼反映出明显更高(P <0.05)比喂食吡ido醇缺乏饮食的鱼的溶菌酶,组织血清蛋白和碱性磷酸酶活性高。然而,每公斤饲料饲喂8 mg吡ido醇的鱼达到了肝脏饱和。生长参数,RNA / DNA比率,SOD,蛋白酶,溶菌酶和肝脏吡ine醇浓度数据相对于不同饮食级吡ido醇水平的折线回归分析表明,其需求量在3.20–6.87 mg / kg的范围内。

更新日期:2021-04-30
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