Global fish consumption is expected to reach 194 million tonnes by 2026 (FAO, 2017). As a result, careful consideration must be given to the environmental impacts of aquaculture expansion, including potential pollution of receiving waters with effluent from the growth and processing of fish products. The main aim of this study was to improve fish processing wastewater treatment by reducing the dissolved inorganic nitrogen concentration and chemical oxygen demand using bioaugmentation, through the application of newly isolated halotolerant, protease-producing bacteria. Five halotolerant bacteria strains were isolated and identified as Marinirhabdus sp., Vibrio sp., Alteromonas sp., Alteromonas macleodii, and Marinobacter hydrocarbonoclasticus based on 16S rRNA sequence phylogenetic analysis. Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus showed significantly higher efficiencies of COD and nitrate, nitrite and ammonium removal compared with the non-bioaugmented control. The COD and nitrogen concentration in fish wastewater treated with Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus were 93 mg L⁻¹, 95 mg L⁻¹, and 66.7 mg L⁻¹, 53.3 mg L⁻¹, respectively, with a decrease in total nitrogen synchronous with the degradation of nitrate, nitrite, and ammonium. This work demonstrated that wastewater bioaugmentation represents an effective treatment for saline, fish processing wastewater, and could be used as a robust method for the removal of organic matter and nitrogen in saline fish wastewater.

到2026年，全球鱼类消费量预计将达到1.94亿吨（粮农组织，2017年）。因此，必须仔细考虑水产养殖扩展对环境的影响，包括鱼类产品的生长和加工所产生的废水对接收水的潜在污染。这项研究的主要目的是通过应用新分离的卤化钾蛋白酶生产细菌，通过生物增强作用降低溶解的无机氮浓度和化学需氧量，从而改善鱼类加工废水的处理。分离出五种卤虫细菌菌株并鉴定为Marinirhabdus sp。，弧菌属sp。，Alteromonas sp。，macleodis alteromonas和基于16S rRNA序列系统发育分析的碳破海洋杆菌。Marinirhabdus sp。与非生物强化对照组相比，碳氢化合物和马立诺杆菌破壁菌显示出更高的COD和硝酸盐，亚硝酸盐和铵盐去除效率。用Marinirhabdus sp。处理的鱼废水中的COD和氮浓度。和Marinobacter hydrocarbonoclasticus分别为93毫克的L ^-1，95毫克的L ^-1，和66.7毫克的L ^-1，53.3毫克的L ^-1总氮的减少与硝酸盐，亚硝酸盐和铵的降解同时发生。这项工作表明废水的生物强化可以有效处理含盐，鱼类加工废水，并且可以用作去除含盐鱼类废水中有机物和氮的可靠方法。