ABSTRACT

The aim of the present work was the comparison of antimicrobial activity, mechanism and components of the ethanol extract (EE) from Lentinula edodes Berk (Pegler) and Agaricus bisporus Sing (Lange). The main component of EE of A. bisporus was dianhydromannitol (20.1%), while isosorbide/dianhydromannitol (21.8%) was detected at a high rate in L. edodes ethanol extract by GC-MS . The common phenolic acids were determined as chlorogenic acid, syringic acid, rutin, p-coumaric acid, ferulic acid, 2-hydroxy cinnamic acid, protocatechuic acid, abscisic acid, and trans-cinnamic acid in both mushroom extract by HPLC-MWD . The MICs (minimum inhibitory concentration) of L. edodes EE on Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Acinetobacter baumannii were between 5.1 mg ml⁻¹ and 6.01 mg ml⁻¹, while MICs of A. bisporus EE on the pathogens were between 5.8 mg ml⁻¹ and 9.54 mg ml⁻¹. The highest DRA decrease was in E. faecalis (69.1%) for L. edodes and S. aureus (71.0%) for A. bisporus in the 20th minute. As a result, L. edodes and A. bisporus have a similar antibacterial effect on the pathogens, and this inhibition effect caused DNA, protein leakage and destruction of permeability of bacterial cell membrane by bioactive molecules in mushroom extract.

摘要

本工作的目的是比较来自香菇(Pegler) 和双孢蘑菇(Lange) 的乙醇提取物 (EE) 的抗菌活性、机制和成分。双孢菌EE的主要成分是双脱水甘露醇（20.1%），而香菇乙醇提取物中异山梨醇/双脱水甘露醇（21.8%）的GC-MS检出率较高。HPLC-MWD测定了两种蘑菇提取物中常见的酚酸为绿原酸、丁香酸、芦丁、对香豆酸、阿魏酸、2-羟基肉桂酸、原儿茶酸、脱落酸和反式肉桂酸。L. edodes EE的 MIC（最低抑菌浓度）肺炎克雷伯菌、金黄色葡萄球菌、粪肠球菌和鲍曼不动杆菌在 5.1 mg ml ^-1和 6.01 mg ml ^-1之间，而双孢杆菌 EE 对病原体的 MIC 在 5.8 mg ml ^-1和 9.54 mg ml ^-1之间。在第 20 分钟内， L. edodes和S. aureus (71.0%) 的DRA 下降最多的是E. faecalis (69.1%) 。结果，L. edodes和A. bisporus对病原体具有相似的抗菌作用，这种抑制作用导致蘑菇提取物中的生物活性分子导致DNA、蛋白质泄漏和细菌细胞膜通透性的破坏。