Pectobacterium carotovorum is a Gram-negative plant-specific pathogen that causes severe rot of postharvest vegetables. Given the safety consideration of pesticides, we developed a peptide approach to address this issue. This hexadecapeptide (zp16) inhibited the growth of planktonic bacterial cells at micromolar concentration and reduced the live bacterial count in a cabbage leaf. Mechanistic studies revealed that zp16 rapidly influenced membrane potential and permeability and triggered significant reactive oxygen species accumulation. Furthermore, proteomics analysis suggested that the expression of some critical proteins related to arginine and peptidoglycan biosynthesis was statistically significantly down-regulated after transitory zp16 treatment. Collectively, zp16 kills the bacteria not only by physically damaging the cellular envelope but also interfering with multiple metabolic processes.

中文翻译：

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合成十六肽通过破坏细胞包膜并抑制精氨酸和肽聚糖的生物合成防止收获后的carotovorum（brasiliensis BC1亚种）感染。

胡萝卜杆菌杆菌是一种革兰氏阴性植物特有的病原体，可导致采后蔬菜严重腐烂。考虑到农药的安全性，我们开发了一种肽方法来解决此问题。这种六肽（zp16）在微摩尔浓度下抑制了浮游细菌细胞的生长，并减少了卷心菜叶中的活细菌数量。机理研究表明，zp16快速影响膜电位和通透性，并引发大量的活性氧积累。此外，蛋白质组学分析表明，在短暂的zp16后，与精氨酸和肽聚糖生物合成有关的某些关键蛋白的表达在统计学上显着下调。治疗。总的来说，zp16不仅通过物理破坏细胞包膜来杀死细菌，而且还干扰了多个代谢过程。