Human intestinal bile salts are well-known for their solubilization and emulsification action in cosmetics, medicines, and chemicals. Here, an effort has been made to understand the solubilization mechanism of bioactive diet capsaicin (Cap) and indole-3-carbinol (I3C) in sodium taurocholate (NaTC) bile salt aggregates using both intrinsic and extrinsic fluorescent probes. Fluorescence study concludes that the NaTC micelle solubilizes both Cap and I3C. An increase in nonpolarity of the NaTC micellar environment is responsible for the solubilization of both Cap and I3C which is further confirmed by using an extrinsic probe Nile red (NR). Both Cap and I3C bind to the NaTC micelle as confirmed from the binding constant value. The location of both the bioactive molecules in the NaTC micelle has been confirmed from fluorescence quenching study using potassium iodide as a quencher. The effect of both I3C and Cap on the size of the NaTC micelle has been analyzed using dynamic light scattering.

中文翻译：

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人肠胆汁盐与生物活性饮食辣椒素和吲哚-3-甲醇的相互作用

人肠胆汁盐以其在化妆品，药​​物和化学药品中的增溶和乳化作用而闻名。在这里，已经尝试使用内在和外在荧光探针来了解生物活性饮食辣椒素（Cap）和吲哚-3-甲醇（I3C）在牛磺胆酸钠（NaTC）胆汁盐聚集物中的增溶机理。荧光研究得出结论，NaTC胶束可溶解Cap和I3C。NaTC胶束环境的非极性增加是Cap和I3C溶解的原因，这可以通过使用外在探针尼罗红（NR）进一步证实。由结合常数值证实，Cap和I3C均与NaTC胶束结合。通过使用碘化钾作为淬灭剂的荧光淬灭研究已证实了两种生物活性分子在NaTC胶束中的位置。使用动态光散射分析了I3C和Cap对NaTC胶束大小的影响。