In this study, we aimed to optimize the nutritional and environmental conditions for the production of a novel lipase (LBL) from Bacillus licheniformis (GenBank accession no. MT118724). This strain was characterized by morphological and biochemical assays and Sanger sequencing of 16S rDNA. The crude lipolytic activity reached a maximum level 7.5 U mL⁻¹ at 40 °C and pH 8.0 using olive oil as substrate. Additionally, the crude enzyme maintained 100% of its initial activity after incubation for 1 h at 50 °C and pH 9.0. It is mandatory to note that LBL lipase displayed appreciable stability over a wide pH range and extreme temperatures. After purification, the optimal lipolytic activity was observed at pH 8.0 and 40 °C. LBL was shown to be a monomeric protein with an estimated molecular weight of 40 kDa. This novel lipase exhibited high stability and excellent compatibility compared to lipase extracted from Thermomyces lanuginosa (Lipolase® from Novozymes, Denmark) toward various detergents. Washing performance analysis revealed that it efficiently removes tomato sauce stain from cotton cloth. All these interesting enzymatic properties favor this new lipase as a potent candidate for applications in detergent formulations.

中文翻译：

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地衣芽孢杆菌脂肪酶的纯化及其作为去色剂添加剂的应用

在这项研究中，我们旨在优化从地衣芽孢杆菌（GenBank 登录号 MT118724）生产新型脂肪酶 (LBL) 的营养和环境条件。该菌株的特征在于形态学和生化分析以及16S rDNA 的Sanger 测序。粗脂肪分解活性达到最高水平 7.5 U mL ^-1在 40 °C 和 pH 8.0 下，使用橄榄油作为底物。此外，粗酶在 50°C 和 pH 9.0 孵育 1 小时后仍保持其初始活性的 100%。必须注意的是，LBL 脂肪酶在很宽的 pH 范围和极端温度下表现出明显的稳定性。纯化后，在 pH 8.0 和 40 °C 下观察到最佳脂肪分解活性。LBL 被证明是一种单体蛋白质，估计分子量为 40 kDa。与从绵状嗜热丝孢菌中提取的脂肪酶相比，这种新型脂肪酶表现出高稳定性和优异的相容性（来自 Novozymes，丹麦的 Lipolase®）用于各种洗涤剂。洗涤性能分析表明，它能有效去除棉布上的番茄酱污渍。所有这些有趣的酶学特性有利于这种新的脂肪酶作为洗涤剂配方应用的有力候选者。