Due to the continuous depletion of non-renewable fossil fuels, there is a focus on renewable energy sources such as bioethanol, biobutanol, biohydrogen and biodiesel. Microalgae have been used to yield high sugar content via alteration of the photosynthetic pathway, thereby enhancing ethanol production. Moreover, certain nanostructured composites in the medium supports biomass enhancement through modification of the photosynthetic pathway. In the present study, reduced graphene oxide-supported platinum-ruthenium (Pt-Ru/RGO) nanoparticles were synthesised, characterised and assessed the role in tris–acetate phosphate (TAP) medium for the improvement of green alga Chlorococcum minutum (C. minutum) biomass under in vitro conditions. Chemically, Pt-Ru/RGO nanoparticles play a useful role as a catalyst in the improvement of chemical reactions and influence the electron supply/transport system. Total chlorophyll and wet biomass contents were 8.26 mg/L and 14.0 g/L in TAP with 1.0 mg/L of nano-Pt-Ru/RGO (CM2) medium when compared with untreated cultures, but total lipid content was more (24.5 g/100 g) in TAP with 0.5 mg/L of nano-Pt-Ru/RGO (CM1) medium. Later, these nano Pt-Ru/RGO-assisted algal feedstocks were used to convert sugars into ethanol by Saccharomyces cerevisiae (yeast) dark fermentation. The current standardised TAP media in the presence of 0.5 or 1.0 mg/L of Pt-Ru/RGO nanoparticles (CM1 or CM2 medium) improved the ethanol production (32.6 and 31.2 g/L at 72 h respectively) from feedstocks of C. minutum. In conclusion, Pt-Ru/RGO nanoparticles can enhance the chemical reactions in photosynthesis likely at the electron transport system and increased the biomass in turn ethanol production.

由于不可再生的化石燃料的不断消耗，人们开始关注可再生能源，例如生物乙醇，生物丁醇，生物氢和生物柴油。微藻已用于通过改变光合作用途径来产生高糖含量，从而提高乙醇产量。而且，培养基中的某些纳米结构复合材料通过光合作用途径的改变来支持生物量的增加。在本研究中，合成了还原性氧化石墨烯负载的铂-钌（Pt-Ru / RGO）纳米颗粒，表征并评估了其在Tris-acetate磷酸盐（TAP）培养基中对绿藻（C. minutum）的改良作用。）体外生物量使适应。在化学上，Pt-Ru / RGO纳米粒子在改善化学反应中起催化剂的作用，并影响电子供应/传输系统。与未经处理的培养物相比，在含1.0 mg / L纳米Pt-Ru / RGO（CM2）培养基的TAP中，叶绿素和湿生物质的总含量为8.26 mg / L和14.0 g / L，但总脂质含量更高（24.5 g在TAP中加入0.5 mg / L的纳米Pt-Ru / RGO（CM1）培养基中的/ 100 g）。后来，这些纳米Pt-Ru / RGO辅助藻类原料被用于通过酿酒酵母（酵母）暗发酵将糖转化为乙醇。当前的标准TAP介质在存在0.5或1.0 mg / L的Pt-Ru / RGO纳米颗粒（CM1或CM2介质）的情况下，提高了C. minutum原料的乙醇产量（分别在72 h时为32.6 g / L和31.2 g / L）。。总之，Pt-Ru / RGO纳米粒子可以增强光合作用中电子传输系统中的化学反应，并增加生物质，进而产生乙醇。