In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6′)-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time.

在过去的十年中，无色杆菌属。与囊性纤维化（CF）患者的慢性定植有关。尽管Achromobacter xylosoxidans是该属中最常见的物种，但在这些患者中也报告了其他物种，例如A. ruhlandii 。无色杆菌中对移动元素的描述很少，而且它们都不是起源于A. ruhlandii。本研究的目的是报告质粒的完整特征，该质粒保存在四个克隆相关的A. ruhlandii分离物中。2013 年至 2015 年间，九个A. ruhlandii在布宜诺斯艾利斯的一家医院，从一名患有 CF 的儿科患者身上发现了分离株。通过 Illumina MiSeq 对四个选定的克隆相关分离株进行测序，使用 RAST 进行注释并手动管理。在四个分离株中观察到存在一个 34096-bp 和 50 个 CDS 的独特质粒，其中只有 1 个核苷酸取代转化为一个氨基酸变化。这些质粒具有包含aac-(6 ' )-Ib基因、汞抗性操纵子区域和relE/stbE毒素/抗毒素系统的 1 类整合子。质粒与来自铜绿假单胞菌的 pmatvim-7 显示出 79% 的相似性和 99% 的同一性。这是第一次完整描述和表征来自A. ruhlandii的质粒随着时间的推移保持不变。