Gastric cancer is a common malignancy in the alimentary system. The laminin subunit gamma 1 (LAMC1) is an important oncogene in human cancers. However, how and whether LAMC1 takes part in gastric cancer progression is largely uncertain. This study analyzed the association between clinical factors of patients and LAMC1 expression and explored the influence of LAMC1 silencing on cell proliferation, migration, invasion, the Warburg effect, protein kinase B (AKT) pathway, and mitogen-activated protein kinase (MEK)/extracellular regulated protein kinase (ERK) pathway in gastric cancer cells. Our results showed LAMC1 abundance was enhanced in gastric cancer samples and cells. LAMC1 was related to the clinical stage, tumor depth, lymph node metastasis, and distant metastasis of patients. LAMC1 silencing inhibited cell proliferation, migration, and invasion. Moreover, LAMC1 knockdown suppressed the Warburg effect via decreasing lactate production, lactate dehydrogenase (LDH) activity, and glucose uptake. LAMC1 interference blocked the activation of the AKT and MEK/ERK signaling. Collectively, LAMC1 knockdown constrained cell proliferation, migration, invasion, and the Warburg effect in gastric cancer cells via inactivating the AKT and MEK/ERK pathway.

中文翻译：

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LAMC1与胃癌患者的不良预后有关，并促进癌细胞恶性肿瘤的发生。

胃癌是消化系统常见的恶性肿瘤。层粘连蛋白亚基γ 1 (LAMC1) 是人类癌症中重要的致癌基因。然而，LAMC1如何以及是否参与胃癌进展在很大程度上是不确定的。本研究分析了患者临床因素与 LAMC1 表达的关系，探讨了 LAMC1 沉默对细胞增殖、迁移、侵袭、Warburg 效应、蛋白激酶 B (AKT) 通路和丝裂原活化蛋白激酶 (MEK) 的影响。胃癌细胞中的细胞外调节蛋白激酶（ERK）通路。我们的结果显示胃癌样本和细胞中的 LAMC1 丰度增加。LAMC1与患者的临床分期、肿瘤深度、淋巴结转移、远处转移有关。LAMC1 沉默抑制细胞增殖、迁移、和入侵。此外，LAMC1 敲低通过降低乳酸产生、乳酸脱氢酶 (LDH) 活性和葡萄糖摄取来抑制 Warburg 效应。LAMC1 干扰阻断了 AKT 和 MEK/ERK 信号的激活。总的来说，LAMC1 敲低通过使 AKT 和 MEK/ERK 通路失活来抑制胃癌细胞中的细胞增殖、迁移、侵袭和 Warburg 效应。