The original PumaPlex is a high-throughput assay developed to genotype 25 single nucleotide polymorphisms (SNPs) in pumas (Puma concolor). Here, we describe the development of PumaPlex100 – an expanded version of the original assay that now genotypes > 100 SNPs. We tested 142 candidate SNPs and developed a panel of 101 polymorphic loci, which are spread across four multiplexes and suitable for genotyping of non-invasive samples. This panel will provide researchers a set of standardized markers, that can be analyzed with minimal bioinformatic skills, for the assessment of population structure and genetic diversity. These SNPs will serve as an important resource for the continued genetic monitoring of this species, especially monitoring through non-invasive sampling.

最初的PumaPlex是一种高通量检测方法，开发用于对美洲狮（Puma concolor）的25个单核苷酸多态性（SNP）进行基因型分析。在这里，我们描述了PumaPlex100的开发-PumaPlex100原始检测的扩展版本，现在的基因型> 100个SNP。我们测试了142个候选SNP，并开发了一个101个多态位点的面板，这些位点分布在四个多重序列中，适用于非侵入性样品的基因分型。该小组将为研究人员提供一套标准化的标记，可以用最少的生物信息学技能对其进行分析，以评估种群结构和遗传多样性。这些单核苷酸多态性将作为继续对该物种进行遗传监测的重要资源，特别是通过无创采样进行的监测。