The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II) enzymes in the presence of glutathione. Recent studies established the presence of unique DJ-1/PfpI domain containing protein named glyoxalase III (Gly III) in prokaryotes, involved in the detoxification of MG into D-lactic acid through a single step process. In the present study, eleven transgenic sugarcane events overexpressing EaGly III were assessed for salinity stress (100 mM and 200 mM NaCl) tolerance. Lipid peroxidation as well as cell membrane injury remained very minimal in all the transgenic events indicating reduced oxidative damage. Transgenic events exhibited significantly higher plant water status, gas exchange parameters, chlorophyll, carotenoid, and proline content, total soluble sugars, SOD and POD activity compared to wild type (WT) under salinity stress. Histological studies by taking the cross section showed a highly stable root system in transgenic events upon exposure to salinity stress. Results of the present study indicate that transgenic sugarcane events overexpressing EaGly III performed well and exhibited improved salinity stress tolerance.

乙二醛酶途径是监测植物中甲基乙二醛 (MG) 水平升高的检查点，在存在谷胱甘肽的情况下由乙二醛酶 I (Gly I) 和乙二醛酶 II (Gly II) 酶介导。最近的研究确定了在原核生物中存在独特的含有 DJ-1/PfpI 结构域的蛋白质，称为乙二醛酶 III (Gly III)，参与通过一步过程将 MG 解毒为 D-乳酸。在本研究中，11 个过表达EaGly III 的转基因甘蔗事件评估了盐胁迫（100 mM 和 200 mM NaCl）耐受性。在所有转基因事件中，脂质过氧化以及细胞膜损伤仍然非常小，表明氧化损伤减少。与盐胁迫下的野生型 (WT) 相比，转基因事件表现出显着更高的植物水分状况、气体交换参数、叶绿素、类胡萝卜素和脯氨酸含量、总可溶性糖、SOD 和 POD 活性。通过横截面进行的组织学研究表明，在暴露于盐分胁迫时，转基因事件中的根系高度稳定。本研究的结果表明，过表达EaGly III 的转基因甘蔗事件表现良好，并表现出改善的盐胁迫耐受性。