The utilization of microalgal biomass for the production of biodiesel and bioethanol is viewed as a promising alternative to increase the offer of both biofuels in the future. In view of this, the autotrophic growth of the green microalga Chlorella vulgaris in a flat-plate photobioreactor was evaluated along with physiological characteristics, such as lipid and carbohydrate composition, and auxiliary parameters of the cultivation using response surface methodology (RSM). Mean biomass productivities of up to 0.262 ± 0.006 g L⁻¹ day⁻¹ were obtained with maximum light intensity and intermediate CO₂ concentrations and the microalgal biomass averaged carbohydrate and lipid contents of 8% and 32% respectively. The light flux supplied to the cultivations and the amount of CO₂ in the feed gas are variables with significant effect on the mean biomass productivity of the microalgae and help estimate the maximum final biomass concentration possible to be obtained. The behavior of associated parameters, such as increase in medium pH and O₂ evolution, was measured and both varied according to different cultivation conditions.

利用微藻生物质生产生物柴油和生物乙醇被认为是未来增加两种生物燃料供应的有前途的替代方法。有鉴于此，评估了绿色微藻小球藻在平板光生物反应器中的自养生长以及生理特性，例如脂质和碳水化合物组成，以及使用响应表面方法（RSM）进行培养的辅助参数。在最大光强度和中等CO _{2的条件下}，获得的平均生物量生产率高达0.262±0.006 g L ^-1  day ^-1浓度和微藻生物质的平均碳水化合物和脂质含量分别为8％和32％。提供给培养物的光通量和进料气中的CO ₂量是对微藻的平均生物量生产率有重大影响的变量，有助于估计可能获得的最大最终生物量浓度。测量了相关参数的行为，例如培养基pH值的增加和O _2的释放，并且都根据不同的培养条件而变化。