Gastric cancer (GC) is one of the most common malignant tumors in the world. As far as we know, no biomarker has been widely accepted for early diagnosis and prognosis prediction of GC. The purpose of this study is to find potential biomarkers to predict the prognosis of GC. The differentially expressed gene (DEG) was analyzed from GSE93774. Enrichr was used to analyze the gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, the enrichment of transcription factors (TF), miRNA, and kinase. GO analysis showed DEGs was enriched in the process of amino acid metabolism. Pathway results showed DEGs was mainly enriched in cell cycle. Propionyl CoA carboxylase alpha (PCCA), Enoyl coenzyme A hydratase short chain 1 (ECHS1), and 3-hydroxyacyl-CoA dehydrogenase (HADH) have prognostic value in patients with GC. ECHS1 and HADH genes were significantly associated with disease-free survival. There was a significant correlation between PCCA and overall survival rate. The results of this study suggest that PCCA, ECHS1, and HADH may be new biomarkers for predicting the prognosis of GC.

胃癌（GC）是世界上最常见的恶性肿瘤之一。据我们所知，目前还没有生物标志物被广泛接受用于GC的早期诊断和预后预测。本研究的目的是寻找预测GC预后的潜在生物标志物。从GSE93774中分析差异表达基因（DEG）。Enrichr用于分析基因本体（GO）、京都基因和基因组百科全书（KEGG）通路、转录因子（TF）、miRNA和激酶的富集。GO分析显示DEGs在氨基酸代谢过程中富集。通路结果显示DEGs主要在细胞周期中富集。丙酰辅酶A羧化酶α（PCCA）、烯酰辅酶A水合酶短链1（ECHS1）和3-羟酰辅酶A脱氢酶（HADH）对GC患者具有预后价值。ECHS1 和 HADH 基因与无病生存显着相关。PCCA与总生存率之间存在显着相关性。本研究结果提示PCCA、ECHS1和HADH可能成为预测GC预后的新生物标志物。