A 60-day feeding trial was conducted to evaluate the effects of dietary taurine (Tau) levels on the growth, biochemical profiles and related immune gene expression of sea cucumbers (Apostichopus japonicus). A total of 225 sea cucumbers (initial body weights 0.79 Â± 0.05 g) were randomly stocked into 5 groups (three tanks per group) and received one of the following diets: Each diet was supplemented with 0 g/kg (T0), 5 g/kg (T0.1), 10 g/kg (T0.5), 20 g/kg (T1) or 50 g/kg (T2) Tau. The sea cucumber weight gain rate, specific growth rate, feed efficiency and crude protein content increased in Tau-supplemented groups compared with T0 group. The body wall total amino acids and total essential amino acids in Tau-supplemented groups were significantly lower than in T0 group while the Tau contents were significantly higher. The acid phosphatase, alkaline phosphatase and complement 3 values were significantly increased in T0.5/T1. Meanwhile, total superoxide dismutase activities in T0.1, T0.5 and T1 were significantly higher than T0 group. The intestinal ATP synthase, Aj-p105, Aj-lys and AjTRX gene expression levels were significantly increased in Tau supplementation groups than in T0 group. And Tau optimum content in the diet of juvenile sea cucumber was determined as 3 g/kg of the dry diet.

中文翻译：

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对幼海参（Apostichopus japonicus）饮食中牛磺酸添加的评估：生长、生化特征和免疫基因表达

一项为期 60 天的饲养试验旨在评估日粮牛磺酸 (Tau) 水平对海参 ( Apostichopus japonicus ) 生长、生化特征和相关免疫基因表达的影响。）。将225只海参（初始体重0.79±0.05 g）随机分为5组（每组3缸），并接受以下饲料之一：每种饲料补充0 g/kg（T0），5 g/kg (T0.1)、10 g/kg (T0.5)、20 g/kg (T1) 或 50 g/kg (T2) Tau。与T0组相比，添加Tau组的海参增重率、比生长率、饲料效率和粗蛋白含量增加。补充Tau组的体壁总氨基酸和总必需氨基酸显着低于T0组，而Tau含量显着高于T0组。酸性磷酸酶、碱性磷酸酶和补体 3 值在 T0.5/T1 中显着增加。同时，T0.1、T0.5和T1组总超氧化物歧化酶活性显着高于T0组。Tau 补充组的Aj -p105、Aj -lys 和Aj TRX 基因表达水平显着高于 T0 组。并确定海参幼鱼饲料中Tau的最适含量为3 g/kg干饲料。