Parsley severe stunt-associated virus (PSSaV) is a recently identified nanovirus first reported in Germany. During a survey for identification of nanoviruses infecting apiaceous plants in south-eastern Iran, PSSaV was identified and characterized using a combination of rolling circle amplification (RCA) and high-throughput sequencing. Parsley plant samples were collected from vegetable production farms in Kerman province. From two symptomatic samples (39Ba and 40Ba), seven PSSaV components (DNA-C, -S, -M, -R, -N, -U1 and -U2) with two phylogenetically distinct variants of DNA-R (R1 and R2) were identified. In common with the German isolate of PSSaV, no DNA-U4 component was identified. In addition, associated alphasatellite molecules were identified in samples 39Ba [n = 6] and 40Ba [n = 5]. Sequence analyses showed that concatenated component sequences of the two Iranian PSSaVs share 97.2% nucleotide identity with each other and 82% to the German isolate. The coat proteins (CPs) of the PSSaV Iranian sequences share 97.2% amino acid identity and ~ 84% identity with that of the German isolate. Sequence and phylogenetic analyses of a total of 11 recovered alphasatellites from the two samples can be classified into the genera Fabenesatellite [n = 2], Milvetsatellite [n = 1], Mivedwarsatellite [n = 2], Subclovsatellite [n = 2], Sophoyesatellite [n = 4] in the family Alphasatellitidae. Identification of PSSaV and other nanoviruses in wild and cultivated plants in Iran reveals that nanoviruses could be causing yield reduction in crops plants in this country.

欧芹严重特技相关病毒 (PSSaV) 是最近在德国首次报道的一种纳米病毒。在鉴定感染伊朗东南部蜂科植物的纳米病毒的调查中，使用滚环扩增 (RCA) 和高通量测序的组合对 PSSaV 进行了鉴定和表征。欧芹植物样本来自克尔曼省的蔬菜生产农场。来自两个有症状的样本（39Ba 和 40Ba）、七个 PSSaV 成分（DNA-C、-S、-M、-R、-N、-U1 和 -U2）以及两个系统发育不同的 DNA-R 变体（R1 和 R2）被识别。与德国的 PSSaV 分离株一样，未鉴定出 DNA-U4 成分。此外，在样品 39Ba [ n  = 6] 和 40Ba [ n = 5]。序列分析表明，两个伊朗 PSSaV 的串联成分序列彼此具有 97.2% 的核苷酸同一性，与德国分离株的核苷酸同一性为 82%。PSSaV 伊朗序列的外壳蛋白 (CP) 与德国分离株具有 97.2% 的氨基酸同一性和约 84% 的同一性。对从两个样品中回收的总共11颗α卫星进行序列和系统发育分析，可分为Fabenesatellite [ n  = 2]、Milvetsatellite [ n  = 1]、Mivedwarsatellite [ n  = 2]、Subclovsatellite [ n  = 2]、Sophoyesatellite [ n = 4] 在Alphasatellitidae科中。在伊朗野生和栽培植物中鉴定出 PSSaV 和其他纳米病毒表明，纳米病毒可能导致该国农作物减产。