UV₂₅₄ irradiation disinfection is a commonly used method to inactivate pathogenic viruses in water and wastewater treatment. Model prediction method can serve as a pre-screening tool to quickly estimate the effectiveness of UV₂₅₄ irradiation on emerging or unculturable viruses. In this study, an improved prediction model was applied to estimate UV₂₅₄ photolysis kinetics of viral genomes (k_{pred, genome}) based on the genome sequences and their photoreactivity and to correlate with the experimental virus infectivity loss kinetics (k_{exp, infectivity}). The UV₂₅₄ inactivation data of 102 viruses (including 2 dsRNA, 65 ssRNA, 33 dsDNA and 2 ssDNA viruses) were collected from the published experimental data with k_{exp, infectivity} ranging from 0.016 to 3.49 cm² mJ⁻¹. The model had fairly good performance in predicting the virus susceptibility to UV₂₅₄ irradiation except dsRNA viruses (Pearson’s correlation coefficient = 0.64) and 70% of k_{pred, genome} fell in the range of 1/2 to 2 times of k_{exp, infectivity}. The positive deviation of the model often occurred for photoresistant viruses with low k_{exp, infectivity} less than 0.20 cm² mJ⁻¹ (e.g., Adenovirus, Papovaviridae and Retroviridae). We also applied this model to predict the UV₂₅₄ inactivation rate of SARS-CoV-2 (k_{pred, genome} = 3.168 cm² mJ⁻¹) and a UV dose of 3 mJ cm⁻² seemed to be able to achieve a 2-log removal by conservative calculation using 1/2k_{pred, genome} value. This prediction method can be used as a prescreening tool to assess the effectiveness of UV₂₅₄ irradiation for emerging/unculturable viruses in water or wastewater treatment.

UV ₂₅₄辐射消毒是在水和废水处理中灭活病原病毒的常用方法。模型预测方法可以用作预筛选工具，以快速估计UV ₂₅₄照射对正在出现的或无法培养的病毒的有效性。在这项研究中，基于基因组序列及其光反应性，使用一种改进的预测模型来估计病毒基因组（k _{pred，基因组}）的UV ₂₅₄光解动力学，并与实验性病毒传染性丧失动力学（k _{exp，传染性}）相关联。紫外线₂₅₄从已发表的实验数据中收集了102种病毒（包括2个dsRNA，65 ssRNA，33 dsDNA和2 ssDNA病毒）的失活数据，其k _{exp的感染力}范围为0.016至3.49 cm ² mJ ^-1。除了dsRNA病毒（Pearson相关系数= 0.64）和k _pred的70％外，该模型在预测病毒对UV ₂₅₄辐射的敏感性方面具有相当好的性能_，基因组落在k _exp感染力的1/2至2倍范围内。该模型的正偏差经常发生在k_值低_，传染性小于0.20 cm ² mJ的光抗性病毒中^-1（例如，腺病毒，巴氏病毒科和逆转录病毒科）。我们还应用了该模型来预测SARS-CoV-2的UV ₂₅₄灭活率（k _{pred，基因组} = 3.168 cm ² mJ ^-1）和3 mJ cm ^-2的UV剂量似乎能够达到2-通过保守计算，使用1/2 k _{pred，基因组}值进行对数去除。该预测方法可以用作预筛选工具，以评估UV ₂₅₄辐射对水或废水处理中新兴/不可培养病毒的有效性。