Annual Review of Physical Chemistry ( IF 14.7 ) Pub Date : 2021-04-20 , DOI: 10.1146/annurev-physchem-061020-053416 Daisy Unsihuay 1 , Daniela Mesa Sanchez 1 , Julia Laskin 1
Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed maps of hundreds of molecules in complex samples with high sensitivity and subcellular spatial resolution. Accurate quantification in MSI relies on a detailed understanding of matrix effects associated with the ionization process along with evaluation of the extraction efficiency and mass-dependent ion losses occurring in the analysis step. We present a critical summary of approaches developed for quantitative MSI of metabolites, lipids, and proteins in biological tissues and discuss their current and future applications.
中文翻译:
生物系统的定量质谱成像
质谱成像 (MSI) 是一种强大的无标记技术,可提供复杂样品中数百个分子的详细图谱,具有高灵敏度和亚细胞空间分辨率。MSI 中的准确定量依赖于对与电离过程相关的基质效应的详细了解,以及对提取效率和分析步骤中发生的质量相关离子损失的评估。我们对为生物组织中代谢物、脂质和蛋白质的定量 MSI 开发的方法进行了重要总结,并讨论了它们当前和未来的应用。